| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Seale, Lucia A. Gilman, Christy L. Moorman, Benjamin P. Berry, Marla J. Grau, E. Gordon Seale, Andre P. |
| Description |
Author Affiliation: Seale LA ( Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96813, USA. Electronic address: lseale@hawaii.edu.); Gilman CL ( Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96813, USA.); Moorman BP ( Hawaii Institute of Marine Biology, University of Hawaii, Kaneohe, HI 96744, USA.); Berry MJ ( Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96813, USA.); Grau EG ( Hawaii Institute of Marine Biology, University of Hawaii, Kaneohe, HI 96744, USA.); Seale AP ( Hawaii Institute of Marine Biology, University of Hawaii, Kaneohe, HI 96744, USA.) |
| Abstract |
Selenoproteins are ubiquitously expressed, act on a variety of physiological redox-related processes, and are mostly regulated by selenium levels in animals. To date, the expression of most selenoproteins has not been verified in euryhaline fish models. The Mozambique tilapia, Oreochromis mossambicus, a euryhaline cichlid fish, has a high tolerance for changes in salinity and survives in fresh water (FW) and seawater (SW) environments which differ greatly in selenium availability. In the present study, we searched EST databases for cichlid selenoprotein mRNAs and screened for their differential expression in FW and SW-acclimated tilapia. The expression of mRNAs encoding iodothyronine deiodinases 1, 2 and 3 (Dio1, Dio2, Dio3), Fep15, glutathione peroxidase 2, selenoproteins J, K, L, M, P, S, and W, was measured in the brain, eye, gill, kidney, liver, pituitary, muscle, and intraperitoneal white adipose tissue. Gene expression of selenophosphate synthetase 1, Secp43, and selenocysteine lyase, factors involved in selenoprotein synthesis or in selenium metabolism, were also measured. The highest variation in selenoprotein and synthesis factor mRNA expression between FW- and SW-acclimated fish was found in gill and kidney. While the branchial expression of Dio3 was increased upon transferring tilapia from SW to FW, the inverse effect was observed when fish were transferred from FW to SW. Protein content of Dio3 was higher in fish acclimated to FW than in those acclimated to SW. Together, these results outline tissue distribution of selenoproteins in FW and SW-acclimated tilapia, and indicate that at least Dio3 expression is regulated by environmental salinity. |
| File Format |
HTM / HTML |
| ISSN |
0946672X |
| e-ISSN |
18783252 |
| DOI |
10.1016/j.jtemb.2014.04.003 |
| Journal |
Journal of Trace Elements in Medicine and Biology |
| Issue Number |
3 |
| Volume Number |
28 |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2014-07-01 |
| Publisher Place |
Germany |
| Access Restriction |
Open |
| Subject Keyword |
Discipline Metabolism Discipline Environmental Health Phosphotransferases Metabolism Selenium Selenoproteins Animals Tilapia Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Research Support, U.s. Gov't, Non-p.h.s. |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Biochemistry Molecular Medicine Inorganic Chemistry |
