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Expression of core-binding factor 1 and osteocalcin in fluoride-treated fibroblasts and osteoblasts.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Duan, Xiaoqin Xu, Hui Wang, Ying Wang, Huan Li, Guangsheng Jing, Ling
Description	Country affiliation: China Author Affiliation: Duan X ( Department of Rehabilitation Medicine of the Second Hospital Norman Bethune of Jilin University, Changchun 130041, China.); Xu H ( Institute of Endemic Disease of Jilin University, 1163 Xinmin Street, Changchun 130021, China.); Wang Y ( The First Hospital Norman Bethune of Jilin University, Changchun 130021, China.); Wang H ( Department of Rehabilitation Medicine of the Second Hospital Norman Bethune of Jilin University, Changchun 130041, China.); Li G ( Institute of Endemic Disease of Jilin University, 1163 Xinmin Street, Changchun 130021, China.); Jing L ( Institute of Endemic Disease of Jilin University, 1163 Xinmin Street, Changchun 130021, China. Electronic address: jingling85619287@163.com.)
Abstract	To study the effects and importance of fluoride on FBs in the development of extraperiosteal calcification and the ossification of skeletal fluorosis, the presence of the osteogenic phenotype, which is indicated by the expression of core-binding factor 1 (Cbfa1) and osteocalcin (OCN), in an FB cell line (L929) and in osteoblasts (OBs) exposed to fluoride was determined. Fibroblasts and osteoblasts were exposed to different concentrations of fluoride (0, 0.0001, 0.001, 0.1, 1.0, 10.0 and 20.0 mg/L F(-)). By using RT-PCR and ELISA, the mRNA levels of Cbfa1 and OCN were measured at 48 h, and the protein levels of Cbfa1 and OCN were measured at 2, 4, 24, 48 and 72 h. The data demonstrated the following: (1) The Cbfa1 protein level in fluoride-treated fibroblasts clearly increased at 48 h in the groups treated with 0.0001, 0.001, 0.1, 1.0 and 20.0 mg/L F(-). The Cbfa1 protein level of the group treated with 10 mg/L F(-) at 72 h was higher than that of the control group. The level of Cbfa1 mRNA in the fibroblasts was much higher at 48 h in the group treated with 10.0 mg/L F(-) than in the control group. (2) The OCN protein level in fluoride-treated fibroblasts was significantly higher than that of the control group in the 0.0001, 0.1, 1.0, 10.0 and 20.0 mg/L F(-) groups at 2 h, and in the 0.001 and 0.1 F(-) groups at 4 h. A slightly higher level of OCN mRNA in fluoride-treated fibroblasts was also found in the 1.0 and 20.0 mg/L F(-) groups compared to the control group. (3) The expressions of Cbfa1 and OCN in osteoblasts treated with the same experimental conditions as the fibroblasts were up-regulated by fluoride following the same trend as in the fibroblasts. Our results showed an increase in the expression of Cbfa1 and OCN in fibroblasts and osteoblasts exposed to fluoride and suggested that the osteogenic function of fibroblasts induced by fluoride could play an important role in the development of extraperiosteal ossification during skeletal fluorosis.
File Format	HTM / HTML
ISSN	0946672X
Issue Number	3
Volume Number	28
e-ISSN	18783252
Journal	Journal of Trace Elements in Medicine and Biology
Language	English
Publisher	Elsevier
Publisher Date	2014-07-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Metabolism Discipline Environmental Health Core Binding Factor Alpha 1 Subunit Metabolism Fibroblasts Osteoblasts Osteocalcin Animals Cell Line Cells, Cultured Enzyme-linked Immunosorbent Assay Drug Effects Fluorides Pharmacology Immunohistochemistry Mice Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Biochemistry Molecular Medicine Inorganic Chemistry

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