NDLI: Lipidtropfen-assoziierte Proteine bei Steatose. Effekte einer Induktion und siRNA-vermittelten Herunterregulation von PAT-Proteinen in hepatozytären Zellkulturmodellen.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Pawella, L. M. Hashani, M. Schirmacher, P. Straub, B. K.
Description	Author Affiliation: Pawella LM ( Pathologisches Institut, Universitätsklinik Heidelberg.)
Abstract	BACKGROUND: The accumulation of hepatocellular lipid droplets, referred to as steatosis, is the most frequent liver pathology in western industrial countries. The surface of lipid droplets is stabilized by amphiphilic proteins of the PAT family (perilipin, adipophilin, TIP47). We recently showed that PAT proteins are differentially expressed in liver. METHODS: The effects of lipid droplet induction with oleate and of siRNA-mediated downregulation of PAT proteins were evaluated using immunofluorescence microscopy, immunoblot and functional assays. RESULTS: Whereas perilipin, adipophilin and (in only minor amounts) also TIP47 coat lipid droplets in steatotic hepatocytes, adipophilin and TIP47, but not perilipin, are detectable in hepatocellular cell cultures. Likewise, perilipin is not induced after downregulation of adipophilin and TIP47. CONCLUSIONS: Common cell culture models show specific differences to human hepatocyte steatosis in vivo. Perilipin may play a role in the long-term storage of fat, which may be only partially reflected by cell culture models.
File Format	HTM / HTML
ISSN	01728113
Issue Number	Suppl 2
Volume Number	31
e-ISSN	14321963
Journal	Der Pathologe
Language	German
Publisher	Springer
Publisher Date	2010-10-01
Publisher Place	Germany
Access Restriction	Subscribed
Subject Keyword	Discipline Pathology Down-regulation Genetics Fatty Liver Phosphoproteins Rna, Small Interfering Carrier Proteins Cell Line Pathology Gene Expression Humans Immunoblotting In Vitro Techniques Lipids Liver Microscopy, Fluorescence Journal Article
Alternative Title	Lipid droplet-associated proteins in steatosis. Effects of induction and siRNA-mediated downregulation of PAT proteins in cell culture models of hepatocyte steatosis
Content Type	Text
Resource Type	Article
Subject	Pathology and Forensic Medicine

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