NDLI: Label-free quantitative analysis of lipid metabolism in living Caenorhabditis elegans.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Le, Thuc T. Duren, Holli M. Slipchenko, Mikhail N. Hu, Chang-Deng Cheng, Ji-Xin
Description	Country affiliation: United States Author Affiliation: Le TT ( Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.)
Abstract	The ubiquity of lipids in biological structures and functions suggests that lipid metabolisms are highly regulated. However, current invasive techniques for lipid studies prevent characterization of the dynamic interactions between various lipid metabolism pathways. Here, we describe a noninvasive approach to study lipid metabolisms using a multifunctional coherent anti-Stokes Raman scattering (CARS) microscope. Using living Caenorhabditis elegans as a model organism, we report label-free visualization of coexisting neutral and autofluorescent lipid species. We find that the relative expression level of neutral and autofluorescent lipid species can be used to assay the genotype-phenotype relationship of mutant C. elegans with deletions in the genes encoding lipid synthesis transcription factors, LDL receptors, transforming growth factor beta receptors, lipid desaturation enzymes, and antioxidant enzymes. Furthermore, by coupling CARS with fingerprint confocal Raman analysis, we analyze the unsaturation level of lipids in wild-type and mutant C. elegans. Our study shows that complex genotype-phenotype relationships between lipid storage, peroxidation, and desaturation can be rapidly and quantitatively analyzed in a single living C. elegans.
File Format	HTM / HTML
ISSN	00222275
e-ISSN	15397262
DOI	10.1194/jlr.D000638
Journal	The Journal of Lipid Research
Issue Number	3
Volume Number	51
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2010-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Biochemistry Caenorhabditis Elegans Metabolism Lipid Metabolism Spectrum Analysis, Raman Animals Genetics Physiology Gene Expression Regulation Lipid Peroxidation Microscopy, Fluorescence Mutation Staining And Labeling Research Support, N.i.h., Extramural Research Support, U.s. Gov't, Non-p.h.s.
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Endocrinology

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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