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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Wang, Meng C. Min, Wei Freudiger, Christian W. Ruvkun, Gary Xie, X. Sunney |
| Description | Country affiliation: United States Author Affiliation: Wang MC ( Department of Molecular and Human Genetics and Huffington Center on Aging, Baylor College of Medicine, Houston, Texas, USA.) |
| Abstract | Identification of genes regulating fat accumulation is important for basic and medical research; genetic screening for those genes in Caenorhabditis elegans, a widely used model organism, requires in vivo quantification of lipids. We demonstrated RNA interference screening based on quantitative imaging of lipids with label-free stimulated Raman scattering (SRS) microscopy, which overcomes major limitations of coherent anti-Stokes Raman scattering microscopy. Our screening yielded eight new genetic regulators of fat storage. |
| File Format | HTM / HTML |
| ISSN | 15487091 |
| e-ISSN | 15487105 |
| DOI | 10.1038/nmeth.1556 |
| Journal | Nature Methods |
| Issue Number | 2 |
| Volume Number | 8 |
| Language | English |
| Publisher | Nature Publishing Group |
| Publisher Date | 2011-02-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Clinical Laboratory Techniques Caenorhabditis Elegans Genetics Fats Gene Expression Regulation Microscopy Rna Interference Spectrum Analysis, Raman Animals Chemistry Metabolism Instrumentation Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology Biotechnology |
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