NDLI: Apolipoprotein M expression increases the size of nascent pre beta HDL formed by ATP binding cassette transporter A1.

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Apolipoprotein M expression increases the size of nascent pre beta HDL formed by ATP binding cassette transporter A1.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Mulya, Anny Seo, Jeongmin Brown, Amanda L. Gebre, Abraham K. Boudyguina, Elena Shelness, Gregory S. Parks, John S.
Description	Country affiliation: United States Author Affiliation: Mulya A ( Department of Pathology/Section on Lipid Sciences, Wake Forest University Health Sciences, Winston-Salem, NC 27157, USA.)
Abstract	Apolipoprotein M (apoM) is a novel apolipoprotein that is reportedly necessary for pre beta HDL formation; however, its detailed function remains unknown. We investigated the biogenesis and properties of apoM and its effects on the initial steps of nascent pre beta HDL assembly by ABCA1 in HEK293 cells. Transiently transfected apoM was localized primarily in the endomembrane compartment. Pulse-chase analyses demonstrated that apoM is inefficiently secreted, relative to human serum albumin, and that approximately 50% remains membrane-associated after extraction with sodium carbonate, pH 11.5. To investigate the role of apoM in nascent pre beta HDL formation, ABCA1-expressing or control cells, transfected with empty vector, apoM, or C-terminal epitope-tagged apoM (apoM-C-FLAG), were incubated with (125)I-apoA-I for 24 h. Conditioned media were harvested and fractionated by fast-protein liquid chromatography (FPLC) to monitor HDL particle size. Pre beta HDL particles were formed effectively in the absence of apoM expression; however, increased apoM expression stimulated the formation of larger-sized nascent pre beta HDLs. Immunoprecipitation with anti-apoA-I antibody followed by apoM Western blot analysis revealed that little secreted apoM was physically associated with pre beta HDL. Our results suggest that apoM is an atypical secretory protein that is not necessary for ABCA1-dependent pre beta HDL formation but does stimulate the formation of larger-sized pre beta HDL. We propose that apoM may function catalytically at an intracellular site to transfer lipid onto pre beta HDL during or after their formation by ABCA1.
File Format	HTM / HTML
ISSN	00222275
e-ISSN	15397262
DOI	10.1194/jlr.M002162
Journal	The Journal of Lipid Research
Issue Number	3
Volume Number	51
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2010-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Biochemistry Atp-binding Cassette Transporters Metabolism Apolipoproteins Gene Expression Regulation High-density Lipoproteins, Pre-beta Chemistry Particle Size Atp Binding Cassette Transporter 1 Amino Acid Sequence Animals Genetics Isolation & Purification Carbonates Cell Line Cloning, Molecular Dna, Complementary Intracellular Space Lipocalins Molecular Sequence Data Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Endocrinology

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Apolipoprotein M expression increases the size of nascent preβ HDL formed by ATP binding cassette transporter A1

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Apolipoprotein M expression increases the size of nascent pre beta HDL formed by ATP binding cassette transporter A1.

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Apolipoprotein M expression increases the size of nascent pre beta HDL formed by ATP binding cassette transporter A1.