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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ichihara, Ken'ichi Fukubayashi, Yumeto |
| Description | Country affiliation: Japan Author Affiliation: Ichihara K ( Biological Chemistry Graduate School of Agricultural Science, Kyoto Prefectural University, Shimogamo, Kyoto 606-8522, Japan. ichihara@kpu.ac.jp) |
| Abstract | A convenient method using commercial aqueous concentrated HCl (conc. HCl; 35%, w/w) as an acid catalyst was developed for preparation of fatty acid methyl esters (FAMEs) from sterol esters, triacylglycerols, phospholipids, and FFAs for gas-liquid chromatography (GC). An 8% (w/v) solution of HCl in methanol/water (85:15, v/v) was prepared by diluting 9.7 ml of conc. HCl with 41.5 ml of methanol. Toluene (0.2 ml), methanol (1.5 ml), and the 8% HCl solution (0.3 ml) were added sequentially to the lipid sample. The final HCl concentration was 1.2% (w/v). This solution (2 ml) was incubated at 45 degrees C overnight or heated at 100 degrees C for 1-1.5 h. The amount of FFA formed in the presence of water derived from conc. HCl was estimated to be <1.4%. The yields of FAMEs were >96% for the above lipid classes and were the same as or better than those obtained by saponification/methylation or by acid-catalyzed methanolysis/methylation using commercial anhydrous HCl/methanol. The method developed here could be successfully applied to fatty acid analysis of various lipid samples, including fish oils, vegetable oils, and blood lipids by GC. |
| File Format | HTM / HTML |
| ISSN | 00222275 |
| e-ISSN | 15397262 |
| DOI | 10.1194/jlr.D001065 |
| Journal | The Journal of Lipid Research |
| Issue Number | 3 |
| Volume Number | 51 |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology |
| Publisher Date | 2010-03-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Biochemistry Esters Chemistry Fatty Acids Chromatography, Liquid Fish Oils Hydrochloric Acid Indicators And Reagents Kinetics Lipids Blood Methanol Methylation Plant Oils Solubility Temperature Toluene |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Endocrinology |
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