NDLI: A stable niche supports long-term maintenance of human epidermal stem cells in organotypic cultures.

Please wait, while we are loading the content...

Lentiviral-mediated HoxB4 expression in human embryonic stem cells initiates early hematopoiesis in a dose-dependent manner but does not promote myeloid differentiation.

Migration of neurotrophic factors-secreting mesenchymal stem cells toward a quinolinic acid lesion as viewed by magnetic resonance imaging.

Directed evolution of motor neurons from genetically engineered neural precursors.

Reprogramming of neural progenitor cells into induced pluripotent stem cells in the absence of exogenous Sox2 expression.

Mesenchymal stem cells alter migratory property of T and dendritic cells to delay the development of murine lethal acute graft-versus-host disease.

Regulation of embryonic stem cell self-renewal and pluripotency by Foxd3.

Characterization of side population cells from human airway epithelium.

Autophagic death of adult hippocampal neural stem cells following insulin withdrawal.

A stable niche supports long-term maintenance of human epidermal stem cells in organotypic cultures.

OP9 Stroma Augments Survival of Hematopoietic Precursors and Progenitors During Hematopoietic Differentiation from Human Embryonic Stem Cells

Enhancing the reliability and throughput of neurosphere culture on hydrogel microwell arrays.

CD34+ testicular stromal cells support long-term expansion of embryonic and adult stem and progenitor cells.

Ex vivo-expanded bone marrow CD34+ derived neutrophils have limited bactericidal ability.

IFATS collection: Adipose stromal cell differentiation is reduced by endothelial cell contact and paracrine communication: role of canonical Wnt signaling.

Allogeneic injection of fetal membrane-derived mesenchymal stem cells induces therapeutic angiogenesis in a rat model of hind limb ischemia.

Ciliary neurotrophic factor-mediated signaling regulates neuronal versus glial differentiation of retinal stem cells/progenitors by concentration-dependent recruitment of mitogen-activated protein kinase and Janus kinase-signal transducer and activator of transcription pathways in conjunction with Notch signaling.

Embryonic Stem Cell Therapy of Heart Failure in Genetic Cardiomyopathy

CD105-positive cells in pulmonary arterial blood of adult human lung cancer patients include mesenchymal progenitors.

Keratinocyte growth factor prevents radiation damage to salivary glands by expansion of the stem/progenitor pool.

Constitutive expression of HIF-1alpha and HIF-2alpha in bone marrow stromal cells differentially promotes their proangiogenic properties.

Putative dental pulp-derived stem/stromal cells promote proliferation and differentiation of endogenous neural cells in the hippocampus of mice.

Adipose stromal/stem cells: basic and translational advances: the IFATS collection.

Year: 2008 Volume: 26 Issue: 9

Year: 2008 Volume: 26 Issue: 8

Year: 2008 Volume: 26 Issue: 7

Year: 2008 Volume: 26 Issue: 6

Year: 2008 Volume: 26 Issue: 5

Year: 2008 Volume: 26 Issue: 4

Year: 2008 Volume: 26 Issue: 3

Year: 2008 Volume: 26 Issue: 2

Year: 2008 Volume: 26 Issue: 1

Year: 2007 Volume: 25

A stable niche supports long-term maintenance of human epidermal stem cells in organotypic cultures.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Muffler, Sonja Stark, Hans-Jürgen Amoros, Mara Falkowska-Hansen, Berit Boehnke, Karsten Bühring, Hans-Jörg Marmé, Alexander Bickenbach, Jackie R. Boukamp, Petra
Description	Country affiliation: Germany Author Affiliation: Muffler S ( Division of Genetics of Skin Carcinogenesis, German Cancer Research Center, Heidelberg, Germany.)
Abstract	Stem cells in human interfollicular epidermis are still difficult to identify, mainly because of a lack of definitive markers and the inability to label human beings for label-retaining cells (LRCs). Here, we report that LRCs could be identified and localized in organotypic cultures (OTCs) made with human cells. Labeling cultures for 2 weeks with iododeoxyuridine (IdU) and then chasing for 6-10 weeks left <1% of basal cells retaining IdU label. Whole mounts demonstrated that LRCs were individually dispersed in the epidermal basal layer. Some LRCs, but not all, colocalized with cells expressing melanoma chondroitin sulfate proteoglycan, a putative stem cell marker. Although we found LRCs in both collagen- and scaffold-based OTCs, only the scaffold-OTCs supported long-term survival and regeneration. LRCs ' short survival in collagen-OTCs was not due to loss of appropriate growth factors from fibroblasts. Instead, it was due to expression of metalloproteinases, especially matrix metalloproteinase (MMP)-2 and MMP-14, which caused collagen fragmentation, matrix degradation, and dislocation of specific basement membrane components bound to epidermal integrins. Blocking MMP activation not only abrogated MMP-dependent matrix degradation but also increased longevity of the epidermis and the LRCs in these cultures. Such findings indicate that the stem cell niche, the microenvironment surrounding and influencing the stem cell, is essential for stem cell survival and function, including long-term tissue regeneration. Disclosure of potential conflicts of interest is found at the end of this article.
File Format	HTM / HTML
ISSN	10665099
Issue Number	10
Volume Number	26
e-ISSN	15494918
Journal	STEM CELLS
Language	English
Publisher	Wiley
Publisher Date	2008-10-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Cell Biology Discipline Embryology Cell Culture Techniques Methods Epidermis Cytology Stem Cell Niche Stem Cells Animals Atrophy Biological Markers Metabolism Bromodeoxyuridine Cell Proliferation Drug Effects Cell Survival Collagen Pharmacology Enzymology Pathology Fibroblasts Gene Expression Regulation Humans Idoxuridine Intercellular Signaling Peptides And Proteins Genetics Matrix Metalloproteinases Rna, Messenger Rats Regeneration Staining And Labeling Time Factors Tissue Scaffolds Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine Molecular Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Boosting and rescuing epidermal superior population from fresh keratinocyte cultures.

Genetically induced cell death in bulge stem cells reveals their redundancy for hair and epidermal regeneration.

Mesenchymal stem cells express epidermal markers in an in vitro reconstructed human skin model

Stem cells of the human epidermis and their niche: composition and function in epidermal regeneration and carcinogenesis

Flow cytometric sorting and analysis of human epidermal stem cell candidates.

In vivo identity of tendon stem cells and the roles of stem cells in tendon healing.

Epidermal cells adhere preferentially to type IV (basement membrane) collagen

Stem cells in asexual reproduction of Enchytraeus japonensis (Oligochaeta, Annelid): proliferation and migration of neoblasts.

Differentiation of embryonic stem cells into fibroblast-like cells in three-dimensional type I collagen gel cultures

A stable niche supports long-term maintenance of human epidermal stem cells in organotypic cultures.

Similar Documents

Boosting and rescuing epidermal superior population from fresh keratinocyte cultures.

Genetically induced cell death in bulge stem cells reveals their redundancy for hair and epidermal regeneration.

Mesenchymal stem cells express epidermal markers in an in vitro reconstructed human skin model

Stem cells of the human epidermis and their niche: composition and function in epidermal regeneration and carcinogenesis

Flow cytometric sorting and analysis of human epidermal stem cell candidates.

In vivo identity of tendon stem cells and the roles of stem cells in tendon healing.

Epidermal cells adhere preferentially to type IV (basement membrane) collagen

Stem cells in asexual reproduction of Enchytraeus japonensis (Oligochaeta, Annelid): proliferation and migration of neoblasts.

Differentiation of embryonic stem cells into fibroblast-like cells in three-dimensional type I collagen gel cultures

A stable niche supports long-term maintenance of human epidermal stem cells in organotypic cultures.