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Characterization of side population cells from human airway epithelium.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hackett, Tillie-Louise Shaheen, Furquan Johnson, Andrew Wadsworth, Samuel Pechkovsky, Dmitri V. Jacoby, David B. Kicic, Anthony Stick, Stephen M. Knight, Darryl A.
Description	Country affiliation: Canada Author Affiliation: Hackett TL ( James Hogg iCAPTURE Centre for Cardiovascular and Pulmonary Disease, St Paul's Hospital, Vancouver, British Columbia, Canada. thackett@mrl.ubc.ca)
Abstract	The airway epithelium is the first line of contact with the inhaled external environment and is continuously exposed to and injured by pollutants, allergens, and viruses. However, little is known about epithelial repair and in particular the identity and role of tissue resident stem/progenitor cells that may contribute to epithelial regeneration. The aims of the present study were to identify, isolate, and characterize side population (SP) cells in human tracheobronchial epithelium. Epithelial cells were obtained from seven nontransplantable healthy lungs and four asthmatic lungs by pronase digestion. SP cells were identified by verapamil-sensitive efflux of the DNA-binding dye Hoechst 33342. Using flow cytometry, CD45(-) SP, CD45(+) SP, and non-SP cells were isolated and sorted. CD45(-) SP cells made up 0.12% +/- 0.01% of the total epithelial cell population in normal airway but 4.1% +/- 0.06% of the epithelium in asthmatic airways. All CD45(-) SP cells showed positive staining for epithelial-specific markers cytokeratin-5, E-cadherin, ZO-1, and p63. CD45(-) SP cells exhibited stable telomere length and increased colony-forming and proliferative potential, undergoing population expansion for at least 16 consecutive passages. In contrast with non-SP cells, fewer than 100 CD45(-) SP cells were able to generate a multilayered and differentiated epithelium in air-liquid interface culture. SP cells are present in human tracheobronchial epithelium, exhibit both short- and long-term proliferative potential, and are capable of generation of differentiated epithelium in vitro. The number of SP cells is significantly greater in asthmatic airways, providing evidence of dysregulated resident SP cells in the asthmatic epithelium. Disclosure of potential conflicts of interest is found at the end of this article.
File Format	HTM / HTML
ISSN	10665099
e-ISSN	15494918
DOI	10.1634/stemcells.2008-0171
Journal	STEM CELLS
Issue Number	10
Volume Number	26
Language	English
Publisher	Wiley
Publisher Date	2008-10-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Cell Biology Discipline Embryology Epithelial Cells Cytology Respiratory System Adolescent Antigens, Cd45 Metabolism Asthma Pathology Bronchi Cell Count Cell Differentiation Cells, Cultured Child, Preschool Colony-forming Units Assay Demography Phenotype Telomere Trachea Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine Molecular Medicine

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