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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Tan, Qi Lui, Pauline Po Yee Lee, Yuk Wa |
| Description | Country affiliation: China Author Affiliation: Tan Q ( 1 Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong , Hong Kong SAR, China .) |
| Abstract | We investigated the spatial distribution of stem cells in tendons and the roles of stem cells in early tendon repair. The relationship between tendon-derived stem cells (TDSCs) isolated in vitro and tendon stem cells in vivo was also explored. Iododeoxyuridine (IdU) label-retaining method was used for labeling stem cells in rat patellar tendons with and without injury. Co-localization of label-retaining cells (LRCs) with different markers was done by immunofluorescent staining. TDSCs were isolated from patellar tendon mid-substance after IdU pulsing, and the expression of different markers in fresh and expanded cells was done by immunofluorescent staining. More LRCs were found at the peritenon and tendon-bone junction compared with the mid-substance. Some LRCs at the peritenon were located at the perivascular niche. The LRC number and the expression of proliferative, tendon-related, pluripotency, and pericyte-related markers in LRCs in the window wound increased. Most of the freshly isolated TDSCs expressed IdU, and some TDSCs expressed pericyte-related markers, which were lost during expansion. Both freshly isolated and subcultured TDSCs expressed pluripotency markers, which were absent in LRCs in intact tendons. In conclusion, we identified LRCs at the peritenon, mid-substance, and tendon-bone junction. There were both vascular and non-vascular sources of LRCs at the peritenon, while the source of LRCs at the mid-substance was non-vascular. LRCs participated in tendon repair via migration, proliferation, activation for tenogenesis, and increased pluripotency. Some LRCs in the window wound were pericyte like. Most of the mid-substance TDSCs were LRCs. The pluripotency markers and pericyte-related marker in LRCs might be important for function after injury. |
| File Format | HTM / HTML |
| ISSN | 15473287 |
| e-ISSN | 15578534 |
| DOI | 10.1089/scd.2013.0073 |
| Journal | Stem Cells and Development |
| Issue Number | 23 |
| Volume Number | 22 |
| Language | English |
| Publisher | Mary Ann Liebert, Inc. |
| Publisher Date | 2013-12-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Cell Biology Discipline Hematology Stem Cells Cytology Tendons Pathology Wound Healing Animals Antigens, Cd44 Metabolism Antigens, Ly Biological Markers Cell Proliferation Idoxuridine Pericytes Pluripotent Stem Cells Rats, Sprague-dawley Staining And Labeling Time Factors Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Developmental Biology Hematology |
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