NDLI: CD34+ testicular stromal cells support long-term expansion of embryonic and adult stem and progenitor cells.

Please wait, while we are loading the content...

Lentiviral-mediated HoxB4 expression in human embryonic stem cells initiates early hematopoiesis in a dose-dependent manner but does not promote myeloid differentiation.

Migration of neurotrophic factors-secreting mesenchymal stem cells toward a quinolinic acid lesion as viewed by magnetic resonance imaging.

Directed evolution of motor neurons from genetically engineered neural precursors.

Reprogramming of neural progenitor cells into induced pluripotent stem cells in the absence of exogenous Sox2 expression.

Mesenchymal stem cells alter migratory property of T and dendritic cells to delay the development of murine lethal acute graft-versus-host disease.

Regulation of embryonic stem cell self-renewal and pluripotency by Foxd3.

Characterization of side population cells from human airway epithelium.

Autophagic death of adult hippocampal neural stem cells following insulin withdrawal.

A stable niche supports long-term maintenance of human epidermal stem cells in organotypic cultures.

OP9 Stroma Augments Survival of Hematopoietic Precursors and Progenitors During Hematopoietic Differentiation from Human Embryonic Stem Cells

Enhancing the reliability and throughput of neurosphere culture on hydrogel microwell arrays.

CD34+ testicular stromal cells support long-term expansion of embryonic and adult stem and progenitor cells.

Ex vivo-expanded bone marrow CD34+ derived neutrophils have limited bactericidal ability.

IFATS collection: Adipose stromal cell differentiation is reduced by endothelial cell contact and paracrine communication: role of canonical Wnt signaling.

Allogeneic injection of fetal membrane-derived mesenchymal stem cells induces therapeutic angiogenesis in a rat model of hind limb ischemia.

Ciliary neurotrophic factor-mediated signaling regulates neuronal versus glial differentiation of retinal stem cells/progenitors by concentration-dependent recruitment of mitogen-activated protein kinase and Janus kinase-signal transducer and activator of transcription pathways in conjunction with Notch signaling.

Embryonic Stem Cell Therapy of Heart Failure in Genetic Cardiomyopathy

CD105-positive cells in pulmonary arterial blood of adult human lung cancer patients include mesenchymal progenitors.

Keratinocyte growth factor prevents radiation damage to salivary glands by expansion of the stem/progenitor pool.

Constitutive expression of HIF-1alpha and HIF-2alpha in bone marrow stromal cells differentially promotes their proangiogenic properties.

Putative dental pulp-derived stem/stromal cells promote proliferation and differentiation of endogenous neural cells in the hippocampus of mice.

Adipose stromal/stem cells: basic and translational advances: the IFATS collection.

Year: 2008 Volume: 26 Issue: 9

Year: 2008 Volume: 26 Issue: 8

Year: 2008 Volume: 26 Issue: 7

Year: 2008 Volume: 26 Issue: 6

Year: 2008 Volume: 26 Issue: 5

Year: 2008 Volume: 26 Issue: 4

Year: 2008 Volume: 26 Issue: 3

Year: 2008 Volume: 26 Issue: 2

Year: 2008 Volume: 26 Issue: 1

Year: 2007 Volume: 25

CD34+ testicular stromal cells support long-term expansion of embryonic and adult stem and progenitor cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kim, Jiyeon Seandel, Marco Falciatori, Ilaria Wen, Duancheng Rafii, Shahin
Description	Country affiliation: United States Author Affiliation: Kim J ( Department of Genetic Medicine, Howard Hughes Medical Institute, Ansary Center for Stem Cell Therapeutics, Weill Medical College of Cornell University, New York, New York 10065, USA.)
Abstract	Stem cells reside in specialized microenvironments created by supporting stromal cells that orchestrate self-renewal and lineage-specific differentiation. However, the precise identity of the cellular and molecular pathways that support self-renewal of stem cells is not known. For example, long-term culture of prototypical stem cells, such as adult spermatogonial stem and progenitor cells (SPCs), in vitro has been impeded by the lack of an optimal stromal cell line that initiates and sustains proliferation of these cells. Indeed, current methods, including the use of mouse embryonic fibroblasts (MEFs), have not been efficient and have generally led to inconsistent results. Here, we report the establishment of a novel CD34-positive cell line, referred to as JK1, derived from mouse testicular stromal cells that not only facilitated long-term SPC culture but also allowed faithful generation of SPCs and multipotent stem cells. SPCs generated on JK1 maintained key features of germ line stem cells, including expression of PLZF, DAZL, and GCNA. Furthermore, these feeders also promoted the long-term cultivation of other types of primitive cells including multipotent adult spermatogonial-derived stem cells, pluripotent murine embryonic stem cells, and embryonic germ cells derived from primordial germ cells. Stem cells could be passaged serially and still maintained expression of characteristic markers such as OCT4 and NANOG in vitro, as well as the ability to generate all three germ layers in vivo. These results indicate that the JK1 cell line is capable of promoting long-term culture of primitive cells. As such, this cell line allows for identification of stromal-derived factors that support long-term proliferation of various types of stem cells and constitutes a convenient alternative to other types of feeder layers. Disclosure of potential conflicts of interest is found at the end of this article.
File Format	HTM / HTML
ISSN	10665099
e-ISSN	15494918
DOI	10.1634/stemcells.2008-0379
Journal	STEM CELLS
Issue Number	10
Volume Number	26
Language	English
Publisher	Wiley
Publisher Date	2008-10-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Cell Biology Discipline Embryology Adult Stem Cells Cytology Antigens, Cd34 Metabolism Embryonic Stem Cells Stromal Cells Testis Actins Animals Cell Line, Transformed Cell Proliferation Colony-forming Units Assay Fibroblasts Mice Mice, Inbred C57bl Multipotent Stem Cells Nih 3t3 Cells Spermatogonia Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine Molecular Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Enumeration of neural stem and progenitor cells in the neural colony-forming cell assay.

Concise review: challenging the pluripotency of human testis-derived ESC-like cells.

Functional assessment of hematopoietic niche cells derived from human embryonic stem cells.

CD34+ Testicular Stromal Cells Support Long-Term Expansion of Embryonic and Adult Stem and Progenitor Cells

Misleading and reliable markers to differentiate between primate testis-derived multipotent stromal cells and spermatogonia in culture

Simulation of an in vitro niche environment that preserves conjunctival progenitor cells.

Embryonic fibroblasts represent a connecting link between mesenchymal and embryonic stem cells.

Cell growth characteristics, differentiation frequency, and immunophenotype of adult ear mesenchymal stem cells.

Genes Involved in Post-Transcriptional Regulation Are Overrepresented in Stem/Progenitor Spermatogonia of Cryptorchid Mouse Testes

CD34+ testicular stromal cells support long-term expansion of embryonic and adult stem and progenitor cells.

Similar Documents

Enumeration of neural stem and progenitor cells in the neural colony-forming cell assay.

Concise review: challenging the pluripotency of human testis-derived ESC-like cells.

Functional assessment of hematopoietic niche cells derived from human embryonic stem cells.

CD34+ Testicular Stromal Cells Support Long-Term Expansion of Embryonic and Adult Stem and Progenitor Cells

Misleading and reliable markers to differentiate between primate testis-derived multipotent stromal cells and spermatogonia in culture

Simulation of an in vitro niche environment that preserves conjunctival progenitor cells.

Embryonic fibroblasts represent a connecting link between mesenchymal and embryonic stem cells.

Cell growth characteristics, differentiation frequency, and immunophenotype of adult ear mesenchymal stem cells.

Genes Involved in Post-Transcriptional Regulation Are Overrepresented in Stem/Progenitor Spermatogonia of Cryptorchid Mouse Testes

CD34+ testicular stromal cells support long-term expansion of embryonic and adult stem and progenitor cells.