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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Pikula, Michal Kondej, Karolina Jaskiewicz, Janusz Skokowski, Jaroslaw Trzonkowski, Piotr |
| Description | Country affiliation: Poland Author Affiliation: Pikula M ( Department of Clinical Immunology and Transplantology, Medical University of Gdansk, Debinki 1, 80211 Gdansk, Poland. pikula@amg.gda.pl) |
| Abstract | ESC (epidermal stem cells) play a central role in the regeneration of human epidermis. These cells are also responsible for wound healing and neoplasm formation. Efficient isolation of ESC allows their use in medicine and pharmacy as well as in basic science. Cultured keratinocytes and ESC may be used as biological dressing in burn injuries, chronic wounds and hereditary disorders. Therefore, the isolation and characterization of ESC have been goals in biomedical science. Here, we present a flow cytometric method for the isolation and analysis of human ESC candidates. The strategy presented for the isolation of ESC combines previously proposed enzymatic digestion and FACS-sorting of the obtained cell suspension that utilizes morphological features, integrin-beta1 expression and Rh123 (Rhodamine 123) accumulation of the cells. We also performed a flow cytometric analysis of sorted cells using a cell tracer. |
| File Format | HTM / HTML |
| ISSN | 10656995 |
| Issue Number | 9 |
| Volume Number | 34 |
| e-ISSN | 10958355 |
| Journal | Cell Biology International |
| Language | English |
| Publisher | Wiley |
| Publisher Date | 2010-09-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Cell Biology Epidermis Cytology Flow Cytometry Methods Stem Cells Antigens, Cd29 Metabolism Biological Markers Cell Proliferation Cell Separation Humans Middle Aged Staining And Labeling Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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