NDLI: Bladder smooth muscle strip contractility as a method to evaluate lower urinary tract pharmacology.

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Bladder smooth muscle strip contractility as a method to evaluate lower urinary tract pharmacology.

Year: 2014 Issue: 89

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Bladder smooth muscle strip contractility as a method to evaluate lower urinary tract pharmacology.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kullmann, F. Aura Daugherty, Stephanie L. de Groat, William C. Birder, Lori A.
Description	Author Affiliation: Kullmann FA ( Department of Medicine, Renal division, University of Pittsburgh School of Medicine); Daugherty SL ( Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine.); de Groat WC ( Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine.); Birder LA ( Department of Medicine, Renal division, University of Pittsburgh School of Medicine)
Abstract	We describe an in vitro method to measure bladder smooth muscle contractility, and its use for investigating physiological and pharmacological properties of the smooth muscle as well as changes induced by pathology. This method provides critical information for understanding bladder function while overcoming major methodological difficulties encountered in in vivo experiments, such as surgical and pharmacological manipulations that affect stability and survival of the preparations, the use of human tissue, and/or the use of expensive chemicals. It also provides a way to investigate the properties of each bladder component (i.e. smooth muscle, mucosa, nerves) in healthy and pathological conditions. The urinary bladder is removed from an anesthetized animal, placed in Krebs solution and cut into strips. Strips are placed into a chamber filled with warm Krebs solution. One end is attached to an isometric tension transducer to measure contraction force, the other end is attached to a fixed rod. Tissue is stimulated by directly adding compounds to the bath or by electric field stimulation electrodes that activate nerves, similar to triggering bladder contractions in vivo. We demonstrate the use of this method to evaluate spontaneous smooth muscle contractility during development and after an experimental spinal cord injury, the nature of neurotransmission (transmitters and receptors involved), factors involved in modulation of smooth muscle activity, the role of individual bladder components, and species and organ differences in response to pharmacological agents. Additionally, it could be used for investigating intracellular pathways involved in contraction and/or relaxation of the smooth muscle, drug structure-activity relationships and evaluation of transmitter release. The in vitro smooth muscle contractility method has been used extensively for over 50 years, and has provided data that significantly contributed to our understanding of bladder function as well as to pharmaceutical development of compounds currently used clinically for bladder management.
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/51807
Journal	Journal of Visualized Experiments
Issue Number	90
Language	English
Publisher	MyJove Corp.
Publisher Date	2014-08-18
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Drug Evaluation, Preclinical Muscle, Smooth Drug Effects Urinary Bladder Animals In Vitro Techniques Muscle Contraction Physiology Rats, Sprague-dawley Research Support, N.i.h., Extramural Video-audio Media
Content Type	Text
Resource Type	Article

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