NDLI: Isolation of blood-vessel-derived multipotent precursors from human skeletal muscle.

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Isolation of blood-vessel-derived multipotent precursors from human skeletal muscle.

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Isolation of blood-vessel-derived multipotent precursors from human skeletal muscle.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Chen, William C. W. Saparov, Arman Corselli, Mirko Crisan, Mihaela Zheng, Bo Péault, Bruno Huard, Johnny
Description	Author Affiliation: Chen WC ( Stem Cell Research Center, Department of Bioengineering and Orthopedic Surgery, University of Pittsburgh); Saparov A ( Department of Orthopedic Surgery, University of Pittsburgh); Corselli M ( Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital and the Orthopaedic Hospital Research Center, University of California at Los Angeles.); Crisan M ( Department of Cell Biology, Erasmus MC Stem Cell Institute.); Zheng B ( OHSU Center for Regenerative Medicine, Oregon Health & Science University.); Péault B ( Centre for Cardiovascular Science and MRC Centre for Regenerative Medicine, Queen's Medical Research Institute and University of Edinburgh); Huard J ( Stem Cell Research Center, Department of Orthopedic Surgery and McGowan Institute for Regenerative Medicine, University of Pittsburgh)
Abstract	Since the discovery of mesenchymal stem/stromal cells (MSCs), the native identity and localization of MSCs have been obscured by their retrospective isolation in culture. Recently, using fluorescence-activated cell sorting (FACS), we and other researchers prospectively identified and purified three subpopulations of multipotent precursor cells associated with the vasculature of human skeletal muscle. These three cell populations: myogenic endothelial cells (MECs), pericytes (PCs), and adventitial cells (ACs), are localized respectively to the three structural layers of blood vessels: intima, media, and adventitia. All of these human blood-vessel-derived stem cell (hBVSC) populations not only express classic MSC markers but also possess mesodermal developmental potentials similar to typical MSCs. Previously, MECs, PCs, and ACs have been isolated through distinct protocols and subsequently characterized in separate studies. The current isolation protocol, through modifications to the isolation process and adjustments in the selective cell surface markers, allows us to simultaneously purify all three hBVSC subpopulations by FACS from a single human muscle biopsy. This new method will not only streamline the isolation of multiple BVSC subpopulations but also facilitate future clinical applications of hBVSCs for distinct therapeutic purposes.
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/51195
Journal	Journal of Visualized Experiments
Issue Number	90
Language	English
Publisher	MyJove Corp.
Publisher Date	2014-08-21
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Adventitia Cytology Endothelial Cells Mesenchymal Stromal Cells Muscle, Skeletal Blood Supply Pericytes Tunica Intima Research Support, Non-u.s. Gov't Research Support, U.s. Gov't, Non-p.h.s. Video-audio Media
Content Type	Text
Resource Type	Article

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