NDLI: Exploring arterial smooth muscle Kv7 potassium channel function using patch clamp electrophysiology and pressure myography.

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Exploring arterial smooth muscle Kv7 potassium channel function using patch clamp electrophysiology and pressure myography.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Brueggemann, Lioubov I. Mani, Bharath K. Haick, Jennifer Byron, Kenneth L.
Description	Country affiliation: United States Author Affiliation: Brueggemann LI ( Department of Molecular Pharmacology & Therapeutics, Loyola University Chicago, USA.)
Abstract	Contraction or relaxation of smooth muscle cells within the walls of resistance arteries determines the artery diameter and thereby controls flow of blood through the vessel and contributes to systemic blood pressure. The contraction process is regulated primarily by cytosolic calcium concentration ([Ca(2+)](cyt)), which is in turn controlled by a variety of ion transporters and channels. Ion channels are common intermediates in signal transduction pathways activated by vasoactive hormones to effect vasoconstriction or vasodilation. And ion channels are often targeted by therapeutic agents either intentionally (e.g. calcium channel blockers used to induce vasodilation and lower blood pressure) or unintentionally (e.g. to induce unwanted cardiovascular side effects). Kv7 (KCNQ) voltage-activated potassium channels have recently been implicated as important physiological and therapeutic targets for regulation of smooth muscle contraction. To elucidate the specific roles of Kv7 channels in both physiological signal transduction and in the actions of therapeutic agents, we need to study how their activity is modulated at the cellular level as well as evaluate their contribution in the context of the intact artery. The rat mesenteric arteries provide a useful model system. The arteries can be easily dissected, cleaned of connective tissue, and used to prepare isolated arterial myocytes for patch clamp electrophysiology, or cannulated and pressurized for measurements of vasoconstrictor/vasodilator responses under relatively physiological conditions. Here we describe the methods used for both types of measurements and provide some examples of how the experimental design can be integrated to provide a clearer understanding of the roles of these ion channels in the regulation of vascular tone.
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/4263
Journal	Journal of Visualized Experiments
Issue Number	67
Language	English
Publisher	MyJove Corp.
Publisher Date	2012-09-14
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Kcnq Potassium Channels Physiology Muscle, Smooth, Vascular Myography Patch-clamp Techniques Animals Rats, Sprague-dawley Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Video-audio Media
Content Type	Text
Resource Type	Article

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