NDLI: Assessment of murine exercise endurance without the use of a shock grid: an alternative to forced exercise.

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Assessment of murine exercise endurance without the use of a shock grid: an alternative to forced exercise.

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Assessment of murine exercise endurance without the use of a shock grid: an alternative to forced exercise.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Conner, Jennifer D. Wolden-Hanson, Tami Quinn, LeBris S.
Description	Author Affiliation: Conner JD ( Research Service, VA Puget Sound Health Care System); Wolden-Hanson T ( Research Service, VA Puget Sound Health Care System.); Quinn LS ( Research Service, VA Puget Sound Health Care System)
Abstract	Using laboratory mouse models, the molecular pathways responsible for the metabolic benefits of endurance exercise are beginning to be defined. The most common method for assessing exercise endurance in mice utilizes forced running on a motorized treadmill equipped with a shock grid. Animals who quit running are pushed by the moving treadmill belt onto a grid that delivers an electric foot shock; to escape the negative stimulus, the mice return to running on the belt. However, avoidance behavior and psychological stress due to use of a shock apparatus can interfere with quantitation of running endurance, as well as confound measurements of postexercise serum hormone and cytokine levels. Here, we demonstrate and validate a refined method to measure running endurance in naïve C57BL/6 laboratory mice on a motorized treadmill without utilizing a shock grid. When mice are preacclimated to the treadmill, they run voluntarily with gait speeds specific to each mouse. Use of the shock grid is replaced by gentle encouragement by a human operator using a tongue depressor, coupled with sensitivity to the voluntary willingness to run on the part of the mouse. Clear endpoints for quantifying running time-to-exhaustion for each mouse are defined and reflected in behavioral signs of exhaustion such as splayed posture and labored breathing. This method is a humane refinement which also decreases the confounding effects of stress on experimental parameters.
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/51846
Journal	Journal of Visualized Experiments
Issue Number	90
Language	English
Publisher	MyJove Corp.
Publisher Date	2014-08-14
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Instrumentation Physical Endurance Physiology Animal Welfare Animals Mice Mice, Inbred C57bl Research Support, N.i.h., Extramural Research Support, U.s. Gov't, Non-p.h.s. Video-audio Media
Content Type	Text
Resource Type	Article

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