NDLI: Stabilization of a raw starch digesting amylase from Aspergillus carbonarius via immobilization on activated and non-activated agarose gel.

Please wait, while we are loading the content...

Cd, Cu, Ni, Mn and Zn resistance and bioaccumulation by thermophilic bacteria, Geobacillus toebii subsp. decanicus and Geobacillus thermoleovorans subsp. stromboliensis

Isolation of a strain of Aspergillus fumigatus able to grow in minimal medium added with an industrial cyanide waste.

Effect of dirhamnolipid on the removal of phenol catalyzed by laccase in aqueous solution.

Production of alkaliphilic, halotolerent, thermostable cellulase free xylanase by Bacillus halodurans PPKS-2 using agro waste: single step purification and characterization.

Novel thermostable lipase from Bacillus circulans IIIB153: comparison with the mesostable homologue at sequence and structure level.

Soil bacterial community composition and diversity respond to cultivation in Karst ecosystems.

Integrated expression of the -amylase, dextranase and glutathione gene in an industrial brewer's yeast strain.

Diversity and composition of the bacterial community of Poyang Lake (China) as determined by 16S rRNA gene sequence analysis.

Production, purification and characterization of laccase from Pleurotus ostreatus grown on tomato pomace.

Timing of malolactic fermentation inoculation in Shiraz grape must and wine: influence on chemical composition.

Expression, purification, and characterization of alanine racemase from Pseudomonas putida YZ-26

Correlation between synthesis variation of 2-alkylquinolones and the antifungal activity of a Burkholderia cepacia strain collection.

Development of a simple and efficient transformation system for the basidiomycetous medicinal fungus Ganoderma lucidum.

Effect of heavy metal-solubilizing microorganisms on zinc and cadmium extractions from heavy metal contaminated soil with Tricholoma lobynsis

Control of potato soft rot caused by Pectobacterium carotovorum and Pectobacterium atrosepticum by Moroccan actinobacteria isolates.

Type 2 IDI performs better than type 1 for improving lycopene production in metabolically engineered E. coli strains.

Technology for efficient and successful delivery of vermicompost colonized bioinoculants in Pogostemon cablin (patchouli) Benth.

Stabilization of a raw starch digesting amylase from Aspergillus carbonarius via immobilization on activated and non-activated agarose gel.

Enhanced phenol degradation by immobilized Acinetobacter sp. strain AQ5NOL 1.

Physiological and antioxidant response by Beauveria bassiana Bals (Vuill.) to different oxygen concentrations

A novel cold active esterase derived from Colombian high Andean forest soil metagenome.

Cordyceps militaris (Hypocreales: Cordycipitaceae): transcriptional analysis and molecular characterization of cox1 and group I intron with putative LAGLIDADG endonuclease.

Degenerate primer design to clone the human repertoire of immunoglobulin heavy chain variable regions.

Natural fruit extracts as non-toxic fluorescent dyes for staining fungal chlamydospores.

Investigation on diversity and population succession dynamics of indigenous bacteria of the maize spermosphere.

Preliminary comparing the toxicities of the hybrid cry1Acs fused with different heterogenous genes provided guidance for the fusion expression of Cry proteins.

Year: 2011 Volume: 27

Year: 2010 Volume: 26

Year: 2007 Volume: 23

Year: 1996 Volume: 12

Year: 1995 Volume: 11

Year: 1994 Volume: 10

Stabilization of a raw starch digesting amylase from Aspergillus carbonarius via immobilization on activated and non-activated agarose gel.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Nwagu, Tochukwu N. Okolo, Bartho N. Aoyagi, Hideki
Description	Country affiliation: Nigeria Author Affiliation: Nwagu TN ( Microbiology Department, Faculty of Biological Sciences, University of Nigeria Nsukka, Nsukka, Nigeria. tochukwu.nwagu@unn.edu.ng)
Abstract	Applications of raw starch digesting amylases (RSDAs) are limited due to instability, product inhibition of enzyme and contamination. RSDA from Aspergillus carbonarius was stabilized through immobilization on agarose gel by adsorption, spontaneous crosslinking and conjugation using glycidol, glutaraldehyde or polyglutaraldehyde. Effects of immobilization on kinetics, catalytic, storage and operational stability of immobilized enzyme were evaluated. Polyglutaraldehyde activated agarose RSDA (PGAg-RSDA) gave the highest immobilization yield (100%) with expressed activity of 86.7% while that of glycidol activated RSDA (GlyAg-RSDA) was 80.4%. A shift in pH from optimum of 5 for the soluble enzyme to 6 for RSDA adsorbed on agarose followed by crosslinking with glutaraldehyde (AgRSDA-CROSS) and simultaneous adsorption and crosslinking (AgRSDA-RET), and pH 7 for PGAg-RSDA was seen. PGAg-RSDA and AgRSDA-CROSS were most pH stable and retained over 82% of their activities between pH 3.5 and 9 compared to 59% for the soluble enzyme. Thermoinactivation studies showed that immobilized RSDAs with the exception of GAg-RSDA retained over 90% of their activities at 60°C for 120 min while soluble enzyme retained only 76% activity under the same condition. AgRSDA-CROSS, PGAg-RSDA, Gly-RSDA and GAg-RSDA retained approximately 100% of their activities after 30 days storage at 4°C. GlyAg-RSDA retained 99.6%, PGAg-RSDA 94%, AgRSDA-CROSS 90%, GAg-RSDA 86.5% and Ag-RSDA-RET 80% activity after 10 batch reactions. Immobilization stabilized RSDA and permits processing at higher temperatures to reduce contamination.
File Format	HTM / HTML
ISSN	09593993
Issue Number	1
Volume Number	28
e-ISSN	15730972
Journal	World Journal of Microbiology and Biotechnology
Language	English
Publisher	Springer
Publisher Date	2012-01-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biotechnology Discipline Microbiology Temperature Sepharose Gels Starch Isolation & Purification Enzyme Stability Enzymes, Immobilized Fungal Proteins Glutaral Metabolism Journal Article Discipline Biotechnology Aspergillus Amylases Chemistry Solanum Tuberosum Hydrogen-ion Concentration Kinetics Enzymology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Stabilization of a raw starch digesting amylase from Aspergillus carbonarius via immobilization on activated and non-activated agarose gel

Chemical modification with phthalic anhydride and chitosan: Viable options for the stabilization of raw starch digesting amylase from Aspergillus carbonarius.

Tuning the catalytic properties of lipases immobilized on divinylsulfone activated agarose by altering its nanoenvironment.

Thermostable -amylase immobilization: Enhanced stability and performance for starch biocatalysis.

Application of maltitol to improve production of raw starch digesting glucoamylase by Aspergillus niger F-08

Raw starch digesting amylase from Thermoactinomyces thalpophilus F13.

Immobilization of -amylase onto a calix[4]arene derivative: Evaluation of its enzymatic activity.

Production and Characteristics of Raw-Starch-Digesting α-Amylase from a Protease-Negative Aspergillus ficum Mutant

Use of mesophilic fungal amylases produced by solid-state fermentation in the cold hydrolysis of raw babassu cake starch.

Stabilization of a raw starch digesting amylase from Aspergillus carbonarius via immobilization on activated and non-activated agarose gel.

Similar Documents

Stabilization of a raw starch digesting amylase from Aspergillus carbonarius via immobilization on activated and non-activated agarose gel

Chemical modification with phthalic anhydride and chitosan: Viable options for the stabilization of raw starch digesting amylase from Aspergillus carbonarius.

Tuning the catalytic properties of lipases immobilized on divinylsulfone activated agarose by altering its nanoenvironment.

Thermostable -amylase immobilization: Enhanced stability and performance for starch biocatalysis.

Application of maltitol to improve production of raw starch digesting glucoamylase by Aspergillus niger F-08

Raw starch digesting amylase from Thermoactinomyces thalpophilus F13.

Immobilization of -amylase onto a calix[4]arene derivative: Evaluation of its enzymatic activity.

Production and Characteristics of Raw-Starch-Digesting α-Amylase from a Protease-Negative Aspergillus ficum Mutant

Use of mesophilic fungal amylases produced by solid-state fermentation in the cold hydrolysis of raw babassu cake starch.

Stabilization of a raw starch digesting amylase from Aspergillus carbonarius via immobilization on activated and non-activated agarose gel.