NDLI: Integrated expression of the -amylase, dextranase and glutathione gene in an industrial brewer's yeast strain.

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Integrated expression of the -amylase, dextranase and glutathione gene in an industrial brewer's yeast strain.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Wang, Jin-Jing Wang, Zhao-Yue He, Xiu-Ping Zhang, Bo-Run
Description	Author Affiliation: Wang JJ ( The Laboratory of Molecular Genetics and Breeding of Yeasts, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People's Republic of China.)
Abstract	Genetic engineering is widely used to meliorate biological characteristics of industrial brewing yeast. But how to solve multiple problems at one time has become the bottle neck in the genetic modifications of industrial yeast strains. In a newly constructed strain TYRL21, dextranase gene was expressed in addition of -amylase to make up -amylase's shortcoming which can only hydrolyze -1,4-glycosidic bond. Meanwhile, 18s rDNA repeated sequence was used as the homologous sequence for an effective and stable expression of LSD1 gene. As a result, TYRL21 consumed about twice much starch than the host strain. Moreover TYRL21 speeded up the fermentation which achieved the maximum cell number only within 3 days during EBC tube fermentation. Besides, flavor evaluation comparing TYRL21 and wild type brewing strain Y31 also confirmed TYRL21's better performances regarding its better saccharides utilization (83% less in residual saccharides), less off-flavor compounds (57% less in diacetyl, 39% less in acetaldehyde, 67% less in pentanedione), and improved stability index (increased by 49%) which correlated with sensory evaluation of final beer product.
File Format	HTM / HTML
ISSN	09593993
Issue Number	1
Volume Number	28
e-ISSN	15730972
Journal	World Journal of Microbiology and Biotechnology
Language	English
Publisher	Springer
Publisher Date	2012-01-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Genomic Instability Research Support, Non-u.s. Gov't Saccharomyces Cerevisiae Proteins Microbiology Alpha-amylases Recombinant Proteins Journal Article Beer Base Sequence Genetics Genes, Fungal Glutathione Industrial Microbiology Discipline Microbiology Gene Expression Dextranase Metabolism Fermentation Carbon Discipline Biotechnology Saccharomyces Cerevisiae Dna, Fungal Genetic Engineering Plasmids Kinetics Mutation
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Applied Microbiology and Biotechnology Biotechnology

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