NDLI: Production, purification and characterization of laccase from Pleurotus ostreatus grown on tomato pomace.

Please wait, while we are loading the content...

Cd, Cu, Ni, Mn and Zn resistance and bioaccumulation by thermophilic bacteria, Geobacillus toebii subsp. decanicus and Geobacillus thermoleovorans subsp. stromboliensis

Isolation of a strain of Aspergillus fumigatus able to grow in minimal medium added with an industrial cyanide waste.

Effect of dirhamnolipid on the removal of phenol catalyzed by laccase in aqueous solution.

Production of alkaliphilic, halotolerent, thermostable cellulase free xylanase by Bacillus halodurans PPKS-2 using agro waste: single step purification and characterization.

Novel thermostable lipase from Bacillus circulans IIIB153: comparison with the mesostable homologue at sequence and structure level.

Soil bacterial community composition and diversity respond to cultivation in Karst ecosystems.

Integrated expression of the -amylase, dextranase and glutathione gene in an industrial brewer's yeast strain.

Diversity and composition of the bacterial community of Poyang Lake (China) as determined by 16S rRNA gene sequence analysis.

Production, purification and characterization of laccase from Pleurotus ostreatus grown on tomato pomace.

Timing of malolactic fermentation inoculation in Shiraz grape must and wine: influence on chemical composition.

Expression, purification, and characterization of alanine racemase from Pseudomonas putida YZ-26

Correlation between synthesis variation of 2-alkylquinolones and the antifungal activity of a Burkholderia cepacia strain collection.

Development of a simple and efficient transformation system for the basidiomycetous medicinal fungus Ganoderma lucidum.

Effect of heavy metal-solubilizing microorganisms on zinc and cadmium extractions from heavy metal contaminated soil with Tricholoma lobynsis

Control of potato soft rot caused by Pectobacterium carotovorum and Pectobacterium atrosepticum by Moroccan actinobacteria isolates.

Type 2 IDI performs better than type 1 for improving lycopene production in metabolically engineered E. coli strains.

Technology for efficient and successful delivery of vermicompost colonized bioinoculants in Pogostemon cablin (patchouli) Benth.

Stabilization of a raw starch digesting amylase from Aspergillus carbonarius via immobilization on activated and non-activated agarose gel.

Enhanced phenol degradation by immobilized Acinetobacter sp. strain AQ5NOL 1.

Physiological and antioxidant response by Beauveria bassiana Bals (Vuill.) to different oxygen concentrations

A novel cold active esterase derived from Colombian high Andean forest soil metagenome.

Cordyceps militaris (Hypocreales: Cordycipitaceae): transcriptional analysis and molecular characterization of cox1 and group I intron with putative LAGLIDADG endonuclease.

Degenerate primer design to clone the human repertoire of immunoglobulin heavy chain variable regions.

Natural fruit extracts as non-toxic fluorescent dyes for staining fungal chlamydospores.

Investigation on diversity and population succession dynamics of indigenous bacteria of the maize spermosphere.

Preliminary comparing the toxicities of the hybrid cry1Acs fused with different heterogenous genes provided guidance for the fusion expression of Cry proteins.

Year: 2011 Volume: 27

Year: 2010 Volume: 26

Year: 2007 Volume: 23

Year: 1996 Volume: 12

Year: 1995 Volume: 11

Year: 1994 Volume: 10

Production, purification and characterization of laccase from Pleurotus ostreatus grown on tomato pomace.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Freixo, Maria do Rosário Karmali, Amin Arteiro, José Maria
Description	Country affiliation: Portugal Author Affiliation: Freixo Mdo R ( Structural Chemistry Research Center of Instituto Superior Técnico of Universidade Técnica de Lisboa, Av. Rovisco Pais, 1100, Lisbon, Portugal.)
Abstract	A strain of Pleurotus ostreatus was grown in tomato pomace as sole carbon source for production of laccase. The culture of P. ostreatus revealed a peak of laccase activity (147 U/L of fermentation broth) on the 4th day of culture with a specific activity of 2.8 U/mg protein. Differential chromatographic behaviour of laccase was investigated on affinity chromatographic matrices containing either urea, acetamide, ethanolamine or IDA as affinity ligands. Laccase exhibited retention on such affinity matrices and it was purified on a Sepharose 6B-BDGE-urea column with final enzyme recoveries of about 60%, specific activity of 6.0 and 18.0 U/mg protein and purification factors in the range of 14-46. It was also possible to demonstrate that metal-free laccase did not adsorb to Sepharose 6B-BDGE-urea column which suggests that adsorption of native laccase on this affinity matrix was apparently due to the specific interaction of carbonyl groups available on the matrix with the active site Cu (II) ions of laccase. The kinetic parameters (V(max), K(m), K(cat), and K(cat)/K(m)) of the purified enzyme for several substrates were determined as well as laccase stability and optimum pH and temperature of enzyme activity. This is the first report describing the production of laccase from P. ostreatus grown on tomato pomace and purification of this enzyme based on affinity matrix containing urea as affinity ligand.
File Format	HTM / HTML
ISSN	09593993
Issue Number	1
Volume Number	28
e-ISSN	15730972
Journal	World Journal of Microbiology and Biotechnology
Language	English
Publisher	Springer
Publisher Date	2012-01-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Research Support, Non-u.s. Gov't Discipline Microbiology Isoenzymes Lycopersicon Esculentum Isolation & Purification Substrate Specificity Biosynthesis Metabolism Fermentation Carbon Industrial Waste Journal Article Discipline Biotechnology Pleurotus Growth & Development Chemistry Chromatography, Affinity Analysis Agriculture Hydrogen-ion Concentration Kinetics Enzymology Copper Laccase
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Production, purification and characterization of laccase from Pleurotus ostreatus grown on tomato pomace

Enzyme Production by Solid Substrate Fermentation of Pleurotus ostreatus and Trametes versicolor on Tomato Pomace

Optimization of laccase production by Pleurotus ostreatus IMI 395545 using the Taguchi DOE methodology

Purification of a thermostable alkaline laccase from papaya (Carica papaya) using affinity chromatography.

Effect of textile dyes on activity and differential regulation of laccase genes from Pleurotus ostreatus grown in submerged fermentation

Enzyme Production by Solid Substrate Fermentation of Pleurotus ostreatus and Trametes versicolor on Tomato Pomace

One-copper laccase-related enzyme from Marasmius sp.: purification, characterization and bleaching of textile dyes.

Characterization of new ß-galactosidase from acidophilic fungus, Teratosphaeria acidotherma AIU BGA-1.

An extracellular laccase with potent dye decolorizing ability from white rot fungus Trametes sp. LAC-01.

Production, purification and characterization of laccase from Pleurotus ostreatus grown on tomato pomace.

Similar Documents

Production, purification and characterization of laccase from Pleurotus ostreatus grown on tomato pomace

Enzyme Production by Solid Substrate Fermentation of Pleurotus ostreatus and Trametes versicolor on Tomato Pomace

Optimization of laccase production by Pleurotus ostreatus IMI 395545 using the Taguchi DOE methodology

Purification of a thermostable alkaline laccase from papaya (Carica papaya) using affinity chromatography.

Effect of textile dyes on activity and differential regulation of laccase genes from Pleurotus ostreatus grown in submerged fermentation

Enzyme Production by Solid Substrate Fermentation of Pleurotus ostreatus and Trametes versicolor on Tomato Pomace

One-copper laccase-related enzyme from Marasmius sp.: purification, characterization and bleaching of textile dyes.

Characterization of new ß-galactosidase from acidophilic fungus, Teratosphaeria acidotherma AIU BGA-1.

An extracellular laccase with potent dye decolorizing ability from white rot fungus Trametes sp. LAC-01.

Production, purification and characterization of laccase from Pleurotus ostreatus grown on tomato pomace.