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Sequence-related amplified polymorphism (SRAP) marker as a new method for identification of endophytic fungi from Taxus.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ren, Na Liu, Jiajia Yang, Dongliang Chen, Jianhua Luan, Mingbao Hong, Juan
Description	Country affiliation: China Author Affiliation: Ren N ( Department of Pharmacy Engineering, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, Hunan, China.)
Abstract	A total of 20 endophytic fungi stains were classified into four groups using traditional morphological identification method, and were studied for genetic diversity by sequence-related amplified polymorphism (SRAP) technique. Genomic DNA (deoxyribonucleic acid) of these strains was extracted with CTAB method. SRAP analysis was done with 24 pairs of primers. All strains could be uniquely distinguished with 584 bands and 446 polymorphism bands which generated 76.4% of polymorphic ratio. Unweighted pair-group method with arithmetical averages cluster analysis enabled construction of a dendrogram for estimating genetic distances between different strains. All strains, which were just divided into four groups by traditional morphology identification, were clustered into four major groups at GS = 0.603 and further separated into eight sub-groups at GS = 0.921. Dendrogram also revealed a large genetic variation in 20 strains; different primer combinations allowed them distinctly distinguished one from others with relatively low genetic similarity. The results show that the SRAP technology is more efficient than traditional morphology identification. It is found that SRAP markers could more really reflect the genetic diversity of endophytic fungi strains from Taxus, and also could be used as a method for identification of endophytic fungi from Taxus. It also suggests that SRAP can be used to establish foundation for further screening of taxol-producing endophytic fungi strains which can produce high levels of paclitaxel.
File Format	HTM / HTML
ISSN	09593993
Issue Number	1
Volume Number	28
e-ISSN	15730972
Journal	World Journal of Microbiology and Biotechnology
Language	English
Publisher	Springer
Publisher Date	2012-01-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Research Support, Non-u.s. Gov't Discipline Microbiology Taxus Microbiology Isolation & Purification Phylogeny Journal Article Discipline Biotechnology Polymorphism, Genetic Fungi Dna, Fungal Base Sequence Classification Endophytes Genetics Mycological Typing Techniques Methods
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Applied Microbiology and Biotechnology Biotechnology

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