NDLI: Autosomal short tandem repeat analysis of ancient DNA by coupled use of mini- and conventional STR kits.

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Autosomal short tandem repeat analysis of ancient DNA by coupled use of mini- and conventional STR kits.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Oh, Chang Seok Lee, Sang Jun Park, Jun Bum Lee, Soong Deok Seo, Seung Bum Kim, Hye Yeon Kim, Jaehyup Kim, Yi-Suk Shin, Dong Hoon
Description	Country affiliation: South Korea Author Affiliation: Oh CS ( Institute of Forensic Medicine, Seoul National University College of Medicine, 28, Yongon-Dong, Chongno-Gu, Seoul 110-799, South Korea.)
Abstract	Multiplex autosomal short tandem repeat (STR) genotyping enables researchers to obtain genetic information from ancient human samples. In this study, we tested newly developed AmpFâSTR(®) MiniFiler™ kit for autosomal STR analysis of ancient DNA (aDNA), using human femurs (n = 8) collected from medieval Korean tombs. After extracting aDNA from the bones, autosomal STR analyses were repeated for each sample using the AmpFâSTR(®) MiniFiler™ and Identifiler™ kits. Whereas only 21.87% of larger-sized loci profiles could be obtained with the Identifiler™ kit, 75% of the same loci profiles were determined by MiniFiler™ kit analysis. This very successful amplification of large-sized STR markers from highly degraded aDNA suggests that the MiniFiler™ kit could be a useful complement to conventional STR kit analysis of ancient samples.
File Format	HTM / HTML
ISSN	00221198
Issue Number	3
Volume Number	57
e-ISSN	15564029
Journal	Journal of Forensic Sciences
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2012-05-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Historical Article Research Support, Non-u.s. Gov't Femur Humans Isolation & Purification History, 16th Century Instrumentation Discipline Forensic Sciences History, 18th Century Genotyping Techniques Journal Article Chemistry Dna Polymerase Chain Reaction Dna Degradation, Necrotic Dna Fingerprinting Microsatellite Repeats Methods
Content Type	Text
Resource Type	Article
Subject	Genetics Pathology and Forensic Medicine

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