NDLI: Enzyme-linked immunosorbent assay for screening dioxin soil contamination by uncontrolled combustion during informal recycling in slums.

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Enzyme-linked immunosorbent assay for screening dioxin soil contamination by uncontrolled combustion during informal recycling in slums.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Trindade, Mirta Nording, Malin Nichkova, Mikaela Spinnel, Erik Haglund, Peter Last, Michael S. Gee, Shirley Hammock, Bruce Last, Jerold A. González-Sapienza, Gualberto Brena, Beatriz M.
Description	Country affiliation: Uruguay Author Affiliation: Trindade M ( Cátedra de Bioquímica, Department of Biosciences, Faculty of Chemistry, University de la República, Montevideo, Uruguay.)
Abstract	Uncontrolled combustion due to garbage recycling is a widespread activity among slum dwellers in distressed economy countries and has been indicated as a major source of dioxin contamination. However, because of the high cost and complexity of gas chromatography/high-resolution mass spectrometry (GC-HRMS) analysis, the magnitude of the problem remains largely unknown. The present study describes a first approach toward the use of a dioxin antibody-based enzyme-linked immunosorbent assay (ELISA) as the basis for a sustainable, simple, and low-cost monitoring program to assess the toxicological impact of uncontrolled combustion in slums. A panel of 16 samples was analyzed by GC-HRMS and ELISA on split extracts. Close to 20% of the analyzed samples showed dioxin concentrations up to almost twice the guidance level for residential soil in several countries, pointing out the need for performing a large-scale monitoring program. Despite the potential for variations in dioxin congener distribution due to the mixed nature of the incinerated material, there was a good correlation between the toxic equivalents as determined by GC-HRMS and ELISA. Furthermore, an interlaboratory ELISA validation showed that the capacity to perform the dioxin ELISA was successfully transferred between laboratories. It was concluded that the ELISA method performed very well as a screening tool to prioritize samples for instrumental analysis, which allows cutting down costs significantly.
File Format	HTM / HTML
ISSN	07307268
e-ISSN	15528618
DOI	10.1897/07-660.1
Journal	Environmental Toxicology and Chemistry
Issue Number	11
Volume Number	27
Language	English
Publisher	Wiley
Publisher Date	2008-11-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Research Support, N.i.h., Extramural Research Support, U.s. Gov't, Non-p.h.s. Soil Pollutants Research Support, Non-u.s. Gov't Discipline Environmental Health Discipline Toxicology Benzofurans Enzyme-linked Immunosorbent Assay Discipline Chemistry Analogs & Derivatives
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Health, Toxicology and Mutagenesis

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ENZYME-LINKED IMMUNOSORBENT ASSAY FOR SCREENING DIOXIN SOIL CONTAMINATION BY UNCONTROLLED COMBUSTION DURING INFORMAL RECYCLING IN SLUMS

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Enzyme-linked immunosorbent assay for screening dioxin soil contamination by uncontrolled combustion during informal recycling in slums.

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ENZYME-LINKED IMMUNOSORBENT ASSAY FOR SCREENING DIOXIN SOIL CONTAMINATION BY UNCONTROLLED COMBUSTION DURING INFORMAL RECYCLING IN SLUMS

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Enzyme-linked immunosorbent assay for screening dioxin soil contamination by uncontrolled combustion during informal recycling in slums.