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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Wei, Lan Wang, Xiaobo Zhang, Peng Sun, Yangyang Jia, Lili Zhao, Jingxin Dong, Shikai Sun, Lixin |
| Description | Country affiliation: China Author Affiliation: Wei L ( School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.); Wang X ( School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.); Zhang P ( Department of Pharmacology, Shenyang Institute for Drug Control, Shenyang 110014, China.); Sun Y ( School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.); Jia L ( School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.); Zhao J ( School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.); Dong S ( School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.); Sun L ( School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address: sunlixin67@yahoo.com.) |
| Abstract | In this study, an ultra performance liquid chromatography tandem mass spectrometry method (UPLC-MS/MS) was developed for simultaneous determination of umbelliferone, apigenin, daphnoretin and genkwanin in total (free and conjugated) forms in rat plasma using psoralen as internal standard. Plasma samples were protein precipitated with acetonitrile followed by liquid-liquid extracted with ethyl acetate. Four ingredients were separated on an Acquity UPLC(®) BEH C18 column using gradient elution with the mobile phase consisting of 0.1% formic acid aqueous solution and acetonitrile, and detected by positive ion electrospray ionization (ESI) in multiple reaction monitoring mode (MRM). The method was linear for all analytes over investigated ranges with all correlation coefficients greater than 0.99. The validated lower limit of quantification was 3ng/mL for umbelliferone, 3ng/mL for apigenin, 12ng/mL for daphnoretin and 2ng/mL for genkwanin, respectively. Intra- and inter-day precisions (RSD%) were less than 15% and accuracy (RE%) ranged from -1.1% to 15%. The mean absolute extraction recoveries of analytes and IS from rat plasma were all more than 75%. The validated method was firstly and successfully applied to investigate the pharmacokinetics of four chemical ingredients after oral administration of Wikstroemia indica extract (WIE) to rats. |
| File Format | HTM / HTML |
| ISSN | 15700232 |
| Volume Number | 1008 |
| e-ISSN | 1873376X |
| Journal | Journal of Chromatography B |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-01-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Chemistry Chromatography, Liquid Methods Coumarins Blood Flavonoids Plant Extracts Analysis Tandem Mass Spectrometry Thymelaeaceae Metabolism Animals Rats Journal Article Research Support, Non-u.s. Gov't Validation Studies |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry |
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