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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Wu, Yun Wang, Fenrong Ai, Yu Ma, Wen Bian, Qiaoxia Lee, David Y-W Dai, Ronghua |
| Description | Country affiliation: China Author Affiliation: Wu Y ( School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.); Wang F ( School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.); Ai Y ( School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.); Ma W ( School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.); Bian Q ( School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.); Lee DY ( Mailman Research Center, McLean Hospital, Harvard Medical School, Boston, MA, United States.); Dai R ( School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address: ronghuadai@sina.com.) |
| Abstract | A simple, sensitive and reliable ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method has been developed and validated for simultaneous quantitation of seven coumarins, the bio-active ingredients of Huo Luo Xiao Ling Dan (HLXLD), in rat plasma. The liquid-liquid extraction method with ether-dichloromethane (2:1, v/v) was used to prepare the plasma samples. Analytes and internal standard (IS) of bifendate were separated on a Shim-pack XR-ODS column (75mm×3.0mm, 2.2µm particles) using gradient elution with the mobile phase consisting of methanol and 0.05% formic acid in water at a flow rate of 0.4mL/min. Detection was performed on a triple quadrupole (TQ) tandem mass spectrometry equipped with an electrospray ionization source in the positive ionization and multiple reaction monitoring (MRM) mode. The lower limits of quantitation (LLOQ) were 0.03-0.25ng/mL for all the analytes. Intra- and inter-day precision and accuracy of the seven analytes were well within acceptance criteria (15%). The matrix effect and the mean extraction recoveries of the analytes and IS from rat plasma were all within satisfaction. The validated method has been successfully applied to compare pharmacokinetic profiles of the seven active ingredients in rat plasma between normal and arthritic rats after oral administration of HLXLD, Angelica pubescens extract and Notopterygium incisum extract, respectively. Results showed that there were remarkable differences in pharmacokinetic properties of the analytes among the different groups. |
| File Format | HTM / HTML |
| ISSN | 15700232 |
| Volume Number | 991 |
| e-ISSN | 1873376X |
| Journal | Journal of Chromatography B |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-06-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Chemistry Anti-inflammatory Agents Pharmacokinetics Chromatography, High Pressure Liquid Methods Coumarins Drugs, Chinese Herbal Tandem Mass Spectrometry Animals Administration & Dosage Blood Arthritis Drug Therapy Limit Of Detection Liquid-liquid Extraction Male Rats Rats, Sprague-dawley Journal Article Research Support, Non-u.s. Gov't Validation Studies |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry |
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