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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Pan, Chenwei Chen, Yongping Chen, Weilai Zhou, Guangyao Jin, Lingxiang Zheng, Yi Lin, Wei Pan, Zhenzhen |
| Description | Country affiliation: China Author Affiliation: Pan C ( The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, China.); Chen Y ( The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.); Chen W ( Wenzhou People's Hospital, Wenzhou 325027, China.); Zhou G ( The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, China.); Jin L ( The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, China.); Zheng Y ( The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, China.); Lin W ( The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, China. Electronic address: linwei196061@163.com.); Pan Z ( The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China. Electronic address: wzzhenzhenpan@126.com.) |
| Abstract | In this work, a rapid and sensitive ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the determination of ledipasvir, sofosbuvir and its metabolite GS-331007 in rat plasma was developed. The analytes and the internal standard (diazepam) were separated on an Acquity UPLC BEH C18 chromatography column (2.1mm×50mm, 1.7µm) using gradient elution with a mobile phase of acetonitrile and 0.1% formic acid in water at a flow rate of 0.4mL/min. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode to monitor the precursor-to-product ion transitions of m/z 889.8â130.1 for ledipasvir, m/z 530.3â243.1 for sofosbuvir, m/z 261.5â113.1 for GS-331007 and m/z 285.2â193.1 for diazepam (IS) using a positive electrospray ionization interface. The method was validated over a concentration range of 2-500ng/mL for ledipasvir, 10-2000ng/mL for sofosbuvir and 10-2000ng/mL for GS-331007. Total time for each chromatography was 3.0min. The intra- and inter-day precision and accuracy of the quality control samples at low, medium, and high concentration levels exhibited relative standard deviations (RSD)<10.2% and the accuracy values ranged from -9.8% to 11.2%. The method was successfully applied to a pharmacokinetic study of ledipasvir, sofosbuvir and GS-331007 in rats. |
| File Format | HTM / HTML |
| ISSN | 15700232 |
| Volume Number | 1008 |
| e-ISSN | 1873376X |
| Journal | Journal of Chromatography B |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-01-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Chemistry Antiviral Agents Blood Benzimidazoles Chromatography, Liquid Methods Fluorenes Sofosbuvir Animals Pharmacokinetics Male Rats Rats, Sprague-dawley Reproducibility Of Results Tandem Mass Spectrometry Journal Article Validation Studies |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry |
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