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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Huang, Yan Li, Jin Xiang, Libing Han, Duo Shen, Xuxia Wu, Xiaohua |
| Description | Country affiliation: China Author Affiliation: Huang Y ( Department of Gynecology, Fudan University Shanghai Cancer Center, 270 Dongan Road, Shanghai 200032, China.) |
| Abstract | OBJECTIVE: Despite evidence that oestrogen may play an important role in the carcinogenesis of cervical cancer, its action and mechanism in cervical cancer invasion are not well defined. STUDY DESIGN: The invasion induced by 17ß-oestradiol (E2) was measured by invasion assay. Real-time polymerase chain reaction (PCR), Western blot, enzyme-linked immunosorbent assay (ELISA) and gelatin zymography were used to study the role of E2 on metastasis-related proteases. The signal pathway was also investigated. RESULTS: E2 was found to significantly enhance the invasion of cervical cell lines including HeLa, CaSki and SiHa cells, but not C33A cells. Moreover, E2 10(-8)M increased the expression and activation of matrix metalloproteinases (MMP-2 and MMP-9) in HeLa and CaSki cells, as shown by real-time PCR, Western blot, ELISA and gelatin zymography. The expression of tissue inhibitor of metalloproteinases (TIMP-1 and TIMP-2) was decreased significantly by E2. Pretreatment with GM6001 10 µM (total MMP inhibitor) or SB-3CT 20 µM (specific gelatinase inhibitor) blocked the pro-invasive effect of E2. E2 was found to induce invasion via the phosphatidylinositol 3-kinase (PI3K) signalling pathway. CONCLUSION: E2 may contribute to cervical cancer metastasis through activation of proteolysis and increased invasion via the PI3K pathway. |
| File Format | HTM / HTML |
| ISSN | 03012115 |
| Issue Number | 2 |
| Volume Number | 165 |
| e-ISSN | 18727654 |
| Journal | European Journal of Obstetrics & Gynecology and Reproductive Biology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-12-01 |
| Publisher Place | Ireland |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Obstetrics Discipline Gynecology Estradiol Pharmacology Neoplasm Invasiveness Physiopathology Neoplasm Metastasis Phosphatidylinositol 3-kinases Physiology Cell Line, Tumor Female Hela Cells Humans Matrix Metalloproteinase 2 Biosynthesis Matrix Metalloproteinase 9 Proteolysis Drug Effects Tissue Inhibitor Of Metalloproteinase-1 Tissue Inhibitor Of Metalloproteinase-2 Uterine Cervical Neoplasms Metabolism Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Reproductive Medicine Obstetrics and Gynecology |
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