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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Jun, Sun-Ae Moon, Chuloo Kang, Cheol-Hee Kong, Sean W. Sang, Byoung-In Um, Youngsoon |
| Description | Country affiliation: South Korea Author Affiliation: Jun SA ( Center for Environmental Technology Research, Korea Institute of Science and Technology, 39-1 Hawolgok-dong, Seongbuk-gu, Seoul 136-791, South Korea.) |
| Abstract | The production of 1,3-propanediol (1,3-PD) was investigated with Klebsiella pneumoniae DSM 4799 using raw glycerol without purification obtained from a biodiesel production process. Fed-batch cultures with suspended cells revealed that 1,3-PD production was more effective when utilizing raw glycerol than pure glycerol (productivity after 47 h of fermentation, 0.84 g L(-1) 1 h(-1) versus 1.51 g L(-1) h(-1) with pure and raw glycerol,respectively). In addition, more than 80 g/L of 1,3-PD was produced using raw glycerol;this is the highest 1,3-PD concentration reported thus far for K. pneumoniae using raw glycerol. Repeated fed-batch fermentation with cell immobilization in a fixed-bed reactor was performed to enhance 1,3-PD production. Production of 1,3-PD increased with the cycle number (1.06 g L(-1) h(-1) versus 1.61 g L(-1) h(-1) at the first and fourth cycle, respectively)due to successful cell immobilization. During 46 cycles of fed-batch fermentation taking place over 1,460 h, a stable and reproducible 1,3-PD production performance was observed with both pure and raw glycerol. Based on our results, repeated fed batch with immobilized cells is an efficient fermentor configuration, and raw glycerol can be utilized to produce 1,3-PD without inhibitory effects caused by accumulated impurities. |
| File Format | HTM / HTML |
| ISSN | 02732289 |
| Issue Number | 1-8 |
| Volume Number | 161 |
| e-ISSN | 15590291 |
| Journal | Applied Biochemistry and Biotechnology |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2010-05-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Discipline Biotechnology Glycerol Metabolism Industrial Microbiology Methods Klebsiella Pneumoniae Propylene Glycols Biofuels Bioreactors Fermentation Cytology Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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