NDLI: Optimization of biodiesel production catalyzed by fungus cells immobilized in fibrous supports.

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Optimization of biodiesel production catalyzed by fungus cells immobilized in fibrous supports.

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Optimization of biodiesel production catalyzed by fungus cells immobilized in fibrous supports.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Chen, Jyh-Ping Lin, Gen-Hsu
Description	Country affiliation: China Author Affiliation: Chen JP ( Department of Chemical and Materials Engineering, Chang Gung University, Kwei-San, Taoyuan 333, Taiwan, Republic of China. jpchen@mail.cgu.edu.tw)
Abstract	A circulating packed-bed bioreactor system using fibrous nonwoven fabric as the immobilization matrix was suitable for simultaneous cell growth and immobilization of Rhizopus oryzae fungus cells, which could be used for lipase-mediated production of biodiesel by methanolysis of soybean oil. Response surface methodology and 5-level-5-factor central composite rotatable design was proved to be a powerful tool for the optimization of methanolysis conditions catalyzed by immobilized R. oryzae whole cell biocatalyst. A quadratic polynomial regression model was used to analyze the relationship between the yield and the significant reaction parameters. The analysis confirmed that water content, molar ratio of methanol to oil, cell weight, and reaction time were the significant factors affecting the yield at a 95% confidence level (p < 0.05). Under the optimum condition at 10.97% (w/w) water content, 0.64 molar ratio of methanol to oil, 2.25% (w/w) cell weight, and 23.3 h reaction time, the predicted value of yield was 72.6%. Validation experiments with yields of 70.77 +/- 2.46% verified the availability and the accuracy of the model.
File Format	HTM / HTML
ISSN	02732289
Issue Number	1-8
Volume Number	161
e-ISSN	15590291
Journal	Applied Biochemistry and Biotechnology
Language	English
Publisher	Springer
Publisher Date	2010-05-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biochemistry Discipline Biotechnology Biofuels Bioreactors Fungal Proteins Metabolism Lipase Rhizopus Catalysis Cell Culture Techniques Models, Theoretical Soybean Oil Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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