NDLI: The family 1 glycoside hydrolase from Clostridium cellulolyticum H10 is a cellodextrin glucohydrolase.

Please wait, while we are loading the content...

Year: 2016 Volume: 180

Year: 2016 Volume: 179

Year: 2016 Volume: 178

Year: 2015 Volume: 177

Year: 2015 Volume: 176

Year: 2015 Volume: 175

Year: 2014 Volume: 174

Year: 2014 Volume: 173

Year: 2014 Volume: 172

Year: 2013 Volume: 171

Year: 2013 Volume: 170

Year: 2013 Volume: 169

Year: 2012 Volume: 168

Year: 2012 Volume: 167

Year: 2012 Volume: 166

Year: 2011 Volume: 165

Year: 2011 Volume: 164

Year: 2011 Volume: 163

Year: 2010 Volume: 162

Year: 2010 Volume: 161

Year: 2010 Volume: 161 Issue: 1-8

Marine diatom, Navicula sp. strain JPCC DA0580 and marine green alga, Chlorella sp. strain NKG400014 as potential sources for biodiesel production.

Evaluation of oxalate decarboxylase and oxalate oxidase for industrial applications.

Production of acetone-butanol-ethanol (ABE) in direct fermentation of cassava by Clostridium saccharoperbutylacetonicum N1-4.

Optimization of biodiesel production catalyzed by fungus cells immobilized in fibrous supports.

Toward plant cell wall degradation under thermophilic condition: a unique microbial community developed originally from swine waste.

Development of real-time PCR primer and probe sets for detecting degenerated and non-degenerated forms of the butanol-producing bacterium Clostridium acetobutylicum ATCC 824.

Ethanol production by fermentation using immobilized cells of Saccharomyces cerevisiae in cashew apple bagasse.

Ethanol production from sugarcane bagasse hydrolysate using Pichia stipitis.

Synthesis of rapeseed biodiesel using short-chained alkyl acetates as acyl acceptor.

Characterization of commercial amylases for the removal of filter cake on petroleum wells.

The family 1 glycoside hydrolase from Clostridium cellulolyticum H10 is a cellodextrin glucohydrolase.

The effect of varying organosolv pretreatment chemicals on the physicochemical properties and cellulolytic hydrolysis of mountain pine beetle-killed lodgepole pine.

Determination of product inhibition of CBH1, CBH2, and EG1 using a novel cellulase activity assay.

Production of sugars and levulinic acid from marine biomass Gelidium amansii.

Directed evolution of a thermophilic beta-glucosidase for cellulosic bioethanol production.

Identification of Saccharomyces cerevisiae genes involved in the resistance to phenolic fermentation inhibitors.

Production of ethanol and feed by high dry matter hydrolysis and fermentation of palm kernel press cake.

Ethanol production from sugarcane bagasse by Zymomonas mobilis using simultaneous saccharification and fermentation (SSF) process.

Chlorella minutissima—A Promising Fuel Alga for Cultivation in Municipal Wastewaters

Screening and Production Study of Microbial Xylanase Producers from Brazilian Cerrado

Development of batch and continuous processes on biodiesel production in a packed-bed reactor by a mixture of immobilized Candida rugosa and Rhizopus oryzae lipases.

Characterisation of specific activities and hydrolytic properties of cell-wall-degrading enzymes produced by Trichoderma reesei Rut C30 on different carbon sources.

Compositional changes in sugarcane bagasse on low temperature, long-term diluted ammonia treatment.

Microbial fed-batch production of 1,3-propanediol using raw glycerol with suspended and immobilized Klebsiella pneumoniae.

Packed-bed reactor running on babassu oil and glycerol to produce monoglycerides by enzymatic route using immobilized Burkholderia cepacia lipase.

Effect of biodiesel-derived raw glycerol on 1,3-propanediol production by different microorganisms.

Performances of Lactobacillus brevis for Producing Lactic Acid from Hydrolysate of Lignocellulosics

Production of lactic acid from sucrose: strain selection, fermentation, and kinetic modeling.

Potential enzyme cost reduction with the addition of surfactant during the hydrolysis of pretreated softwood.

Production of cellulase from kraft paper mill sludge by Trichoderma reesei rut C-30.

Phenotype microarray profiling of Zymomonas mobilis ZM4.

Ethanol production from the organic fraction obtained after thermal pretreatment of municipal solid waste.

Nitrogen source optimization for cellulase production by Penicillium funiculosum, using a sequential experimental design methodology and the desirability function.

Production of biodiesel via enzymatic ethanolysis of the sunflower and soybean oils: modeling.

Evaluation of enzyme mixtures in releasing fermentable sugars from pre-pulping extracts of mixed northeast hardwoods.

Optimizing Dilute-Acid Pretreatment of Rapeseed Straw for Extraction of Hemicellulose

Optimization of lactic acid production by pellet-form Rhizopus oryzae in 3-L airlift bioreactor using response surface methodology.

Bioconversion of Kraft Paper Mill Sludges to Ethanol by SSF and SSCF

Immobilization and Stabilization of Xylanase by Multipoint Covalent Attachment on Agarose and on Chitosan Supports

Partitioning of porcine pancreatic lipase in a two-phase systems of polyethylene glycol/potassium phosphate aqueous.

beta-D-Xylosidase from Selenomonas ruminantium: role of glutamate 186 in catalysis revealed by site-directed mutagenesis, alternate substrates, and active-site inhibitor.

Maximizing algal growth in batch reactors using sequential change in light intensity.

Production of cellulolytic enzymes by fungi Acrophialophora nainiana and Ceratocystis paradoxa using different carbon sources.

Empirical evaluation of inhibitory product, substrate, and enzyme effects during the enzymatic saccharification of lignocellulosic biomass.

Dilute ammonia pretreatment of sorghum and its effectiveness on enzyme hydrolysis and ethanol fermentation.

Light regime characterization in an airlift photobioreactor for production of microalgae with high starch content.

Biodiesel production from integration between reaction and separation system: reactive distillation process.

Year: 2010 Volume: 160

Year: 2009 Volume: 159

Year: 2009 Volume: 158

Year: 2009 Volume: 156

Year: 2009 Volume: 155

Year: 2009 Volume: 154

Year: 2009 Volume: 153

Year: 2008 Volume: 151

Year: 2008 Volume: 150

Year: 2008 Volume: 149

Year: 2008 Volume: 148

Year: 2008 Volume: 146

Year: 2008 Volume: 145

Year: 2008 Volume: 144

Year: 2007 Volume: 137-140

Year: 2006 Volume: 131

Year: 1982 Volume: 7

Year: 1981 Volume: 6

The family 1 glycoside hydrolase from Clostridium cellulolyticum H10 is a cellodextrin glucohydrolase.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Liu, Wenjin Bevan, David R. Zhang, Y-H Percival
Description	Country affiliation: United States Author Affiliation: Liu W ( Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, 210-A Seitz Hall, Blacksburg, VA 24061, USA.)
Abstract	The only family 1 glycoside hydrolase in Clostridium cellulolyticum H10 (CcGH1) is annotated as a beta-galactosidase but has high sequence homology with many beta-glucosidases. Given the possible importance of beta-glucosidase in cellulose utilization by C. cellulolyticum, the encoding open reading frame Ccel_0374 was cloned and expressed in E. coli as a soluble fusion protein with thioredoxin. After tag cleavage, the purified enzyme had a molecular mass of 52 kDa and was active in dimeric form on a broad range of substrates, including cellobiose, cellotriose, cellotetraose, p-nitrophenyl-beta-glucopyranoside, lactose, and o-nitrophenyl-beta-galactopyranoside. The enzyme showed lower K(m) and higher catalytic efficiency (k (cat)/K(m)) on cellodextrins with degree of polymerization from 2 to 4 than on lactose, and the k (cat)/K (m) values on cellodextrins increased in the order of cellobiose < cellotriose < cellotetraose, suggesting that CcGH1 was a cellodextrin glucohydrolase (EC 3.2.1.74). The high K(m) (69 mM) on cellobiose implies that CcGH1 likely has a minimal role in the intracellular hydrolysis of cellobiose in C. cellulolyticum. The three-dimensional structure model of CcGH1 generated by homology modeling showed a typical (alpha/beta)(8) barrel topology characteristic of family 1 glycoside hydrolases.
File Format	HTM / HTML
ISSN	02732289
Issue Number	1-8
Volume Number	161
e-ISSN	15590291
Journal	Applied Biochemistry and Biotechnology
Language	English
Publisher	Springer
Publisher Date	2010-05-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biochemistry Discipline Biotechnology Bacterial Proteins Metabolism Cellulose Analogs & Derivatives Clostridium Cellulolyticum Enzymology Dextrins Glycoside Hydrolases Chemistry Genetics Models, Molecular Molecular Sequence Data Open Reading Frames Protein Structure, Tertiary Recombinant Fusion Proteins Substrate Specificity Journal Article Research Support, Non-u.s. Gov't Research Support, U.s. Gov't, Non-p.h.s.
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in