NDLI: Rapid and accurate liquid chromatography and tandem mass spectrometry method for the simultaneous quantification of ten metabolic reactions catalyzed by hepatic cytochrome P450 enzymes.

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Rapid and accurate liquid chromatography and tandem mass spectrometry method for the simultaneous quantification of ten metabolic reactions catalyzed by hepatic cytochrome P450 enzymes.

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Rapid and accurate liquid chromatography and tandem mass spectrometry method for the simultaneous quantification of ten metabolic reactions catalyzed by hepatic cytochrome P450 enzymes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Shi, Rong Ma, Bingliang Wu, Jiasheng Wang, Tianming Ma, Yueming
Description	Country affiliation: China Author Affiliation: Shi R ( Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai, China.); Ma B ( Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai, China.); Wu J ( Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai, China.); Wang T ( Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai, China.); Ma Y ( Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai, China.)
Abstract	The hepatic cytochrome P450 enzymes play a central role in the biotransformation of endogenous and exogenous substances. A sensitive high-throughput liquid chromatography with tandem mass spectrometry assay was developed and validated for the simultaneous quantification of the products of ten metabolic reactions catalyzed by hepatic cytochrome P450 enzymes. After the substrates were incubated separately, the samples were pooled and analyzed by liquid chromatography with tandem mass spectrometry using an electrospray ionization source in the positive and negative ion modes. The method exhibited linearity over a broad concentration range, insensitivity to matrix effects, and high accuracy, precision, and stability. The novel method was successfully applied to study the kinetics of phenacetin-O deethylation, coumarin-7 hydroxylation, bupropion hydroxylation, taxol-6 hydroxylation, omeprazole-5 hydroxylation, dextromethorphan-O demethylation, tolbutamide-4 hydroxylation, chlorzoxazone-6 hydroxylation, testosterone-6ß hydroxylation, and midazolam-1 hydroxylation in rat liver microsomes.
File Format	HTM / HTML
ISSN	16159306
Issue Number	19
Volume Number	38
e-ISSN	16159314
Journal	Journal of Separation Science
Language	English
Publisher	Wiley-VCH
Publisher Date	2015-10-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Analytical Discipline Chemistry Chromatography, Liquid Methods Cytochrome P-450 Enzyme System Metabolism Microsomes, Liver Enzymology Tandem Mass Spectrometry Animals Statistics & Numerical Data Humans Hydroxylation In Vitro Techniques Kinetics Male Rats Rats, Sprague-dawley Spectrometry, Mass, Electrospray Ionization Substrate Specificity Journal Article Research Support, Non-u.s. Gov't Validation Studies
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Filtration and Separation

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