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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kazarjan, Jana Mahlapuu, Riina Hansen, Mats Soomets, Ursel Kaljurand, Mihkel Vaher, Merike |
| Description | Country affiliation: Estonia Author Affiliation: Kazarjan J ( Department of Chemistry, Tallinn University of Technology, Tallinn, Estonia.); Mahlapuu R ( Institute of Biomedicine and Translational Medicine, Department of Biochemistry, Centre of Excellence for Translational Medicine, University of Tartu, Tartu, Estonia.); Hansen M ( Institute of Biomedicine and Translational Medicine, Department of Biochemistry, Centre of Excellence for Translational Medicine, University of Tartu, Tartu, Estonia.); Soomets U ( Institute of Biomedicine and Translational Medicine, Department of Biochemistry, Centre of Excellence for Translational Medicine, University of Tartu, Tartu, Estonia.); Kaljurand M ( Department of Chemistry, Tallinn University of Technology, Tallinn, Estonia.); Vaher M ( Department of Chemistry, Tallinn University of Technology, Tallinn, Estonia.) |
| Abstract | In the present study, a micellar electrokinetic chromatographic method was used to determine the retention factors of hydrophilic monomeric and homodimeric forms of glutathione analogues. Ionic-liquid-based surfactant, 1-tetradecyl-3-methylimidazolium chloride, as well as cetyltrimethylammonium bromide and phosphate buffer (pH 7.4) were employed in the experiments. Since the studied peptides possess a negative charge under physiological conditions, it is expected that the peptides interact with the oppositely charged 1-tetradecyl-3-methylimidazolium chloride and cetyltrimethylammonium bromide micelles via hydrophobically assisted electrostatic forces. The dependence of the retention factor on the micellar concentration of 1-tetradecyl-3-methylimidazolium chloride and cetyltrimethylammonium bromide is nonlinear and the obtained curves converge to a limiting value. The retention factor values of GSH analogues were in the range of 0.36-2.22 for glutathione analogues and -1.21 to 0.37 for glutathione when 1-tetradecyl-3-methylimidazolium chloride was used. When cetyltrimethylammonium bromide was employed, the retention factor values were in the range of 0.27-2.17 for glutathione analogues and -1.22 to 0.06 for glutathione. If sodium dodecyl sulfate was used, the retention factor values of glutathione analogues with carnosine moiety were in the range of -1.54 to 0.38. |
| File Format | HTM / HTML |
| ISSN | 16159306 |
| Issue Number | 19 |
| Volume Number | 38 |
| e-ISSN | 16159314 |
| Journal | Journal of Separation Science |
| Language | English |
| Publisher | Wiley-VCH |
| Publisher Date | 2015-10-01 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Chromatography, Micellar Electrokinetic Capillary Methods Glutathione Analogs & Derivatives Analysis Antioxidants Cetrimonium Compounds Humans Hydrophobic And Hydrophilic Interactions Imidazoles Ionic Liquids Micelles Oligopeptides Sodium Dodecyl Sulfate Surface-active Agents Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Analytical Chemistry Filtration and Separation |
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