| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Song, Wei Qiao, Xue Liang, Wen-fei Ji, Shuai Yang, Lu Wang, Yuan Xu, Yong-wei Yang, Ying Guo, De-an Ye, Min |
| Description |
Country affiliation: China Author Affiliation: Song W ( State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China.); Qiao X ( State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China.); Liang WF ( State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China.); Ji S ( State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China.); Yang L ( State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China.); Wang Y ( State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China.); Xu YW ( Waters Corporation, Pudong New District, Shanghai, China.); Yang Y ( Waters Corporation, Pudong New District, Shanghai, China.); Guo DA ( State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China.); Ye M ( State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.) |
| Abstract |
Curcumin is the major constituent of turmeric (Curcuma longa L.). It has attracted widespread attention for its anticancer and anti-inflammatory activities. The separation of curcumin and its two close analogs, demethoxycurcumin and bisdemethoxycurcumin, has been challenging by conventional techniques. In this study, an environmentally friendly method based on supercritical fluid chromatography was established for the rapid and facile separation of the three curcuminoids directly from the methanol extract of turmeric. The method was first developed and optimized by ultra performance convergence chromatography, and was then scaled up to preparative supercritical fluid chromatography. Eluted with supercritical fluid $CO_{2}$ containing 8–15% methanol (containing 10 mM oxalic acid) at a flow rate of 80 mL/min, curcumin, demethoxycurcumin and bisdemethoxycurcumin could be well separated on a Viridis BEH OBD column (Waters, 250 mm × 19 mm, 5 μm) within 6.5 min. As a result, 20.8 mg of curcumin (97.9% purity), 7.0 mg of demethoxycurcumin (91.1%), and 4.6 mg of bisdemethoxycurcumin (94.8%) were obtained after a single step of supercritical fluid chromatography separation with a mean recovery of 76.6%. Showing obvious advantages in low solvent consumption, large sample loading, and easy solvent removal, supercritical fluid chromatography was proved to be a superior technique for the efficient separation of natural products. |
| File Format |
HTM / HTML |
| ISSN |
16159306 |
| Issue Number |
19 |
| Volume Number |
38 |
| e-ISSN |
16159314 |
| Journal |
Journal of Separation Science |
| Language |
English |
| Publisher |
Wiley-VCH |
| Publisher Date |
2015-10-01 |
| Publisher Place |
Germany |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Analytical Discipline Chemistry Chromatography, Supercritical Fluid Methods Curcuma Chemistry Curcumin Analogs & Derivatives Isolation & Purification Anti-inflammatory Agents, Non-steroidal Antineoplastic Agents Chromatography, High Pressure Liquid Humans Solvents Journal Article Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Analytical Chemistry Filtration and Separation |
