NDLI: Effects of spaceflight on murine skeletal muscle gene expression

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Allen, David L. Bandstra, Eric R. Harrison, Brooke C. Thorng, Seiha Stodieck, Louis S. Kostenuik, Paul J. Morony, Sean Lacey, David L. Hammond, Timothy G. Leinwand, Leslie L. Argraves, W. Scott Bateman, Ted A. Barth, Jeremy L.
Description	Country affiliation: United States Author Affiliation: Allen DL ( Department of Integrative Physiology, University of Colorado, Boulder, Colorado, USA.)
Abstract	Spaceflight results in a number of adaptations to skeletal muscle, including atrophy and shifts toward faster muscle fiber types. To identify changes in gene expression that may underlie these adaptations, we used both microarray expression analysis and real-time polymerase chain reaction to quantify shifts in mRNA levels in the gastrocnemius from mice flown on the 11-day, 19-h STS-108 shuttle flight and from normal gravity controls. Spaceflight data also were compared with the ground-based unloading model of hindlimb suspension, with one group of pure suspension and one of suspension followed by 3.5 h of reloading to mimic the time between landing and euthanization of the spaceflight mice. Analysis of microarray data revealed that 272 mRNAs were significantly altered by spaceflight, the majority of which displayed similar responses to hindlimb suspension, whereas reloading tended to counteract these responses. Several mRNAs altered by spaceflight were associated with muscle growth, including the phosphatidylinositol 3-kinase regulatory subunit p85alpha, insulin response substrate-1, the forkhead box O1 transcription factor, and MAFbx/atrogin1. Moreover, myostatin mRNA expression tended to increase, whereas mRNA levels of the myostatin inhibitor FSTL3 tended to decrease, in response to spaceflight. In addition, mRNA levels of the slow oxidative fiber-associated transcriptional coactivator peroxisome proliferator-associated receptor (PPAR)-gamma coactivator-1alpha and the transcription factor PPAR-alpha were significantly decreased in spaceflight gastrocnemius. Finally, spaceflight resulted in a significant decrease in levels of the microRNA miR-206. Together these data demonstrate that spaceflight induces significant changes in mRNA expression of genes associated with muscle growth and fiber type.
File Format	HTM / HTML
ISSN	87507587
e-ISSN	15221601
DOI	10.1152/japplphysiol.90780.2008
Journal	Journal of Applied Physiology
Issue Number	2
Volume Number	106
Language	English
Publisher	American Physiological Society
Publisher Date	2009-02-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physiology Gene Expression Regulation Muscle, Skeletal Metabolism Muscular Atrophy Genetics Space Flight Weightlessness Adaptation, Physiological Animals Cluster Analysis Gene Expression Profiling Hindlimb Suspension Mice Mice, Inbred C57bl Micrornas Muscle Fibers, Skeletal Physiopathology Myostatin Oligonucleotide Array Sequence Analysis Phosphatidylinositol 3-kinases Polymerase Chain Reaction Protein Kinases Proto-oncogene Proteins C-akt Rna, Messenger Reproducibility Of Results Tor Serine-threonine Kinases Time Factors Comparative Study Research Support, N.i.h., Extramural Research Support, U.s. Gov't, Non-p.h.s.
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Sports Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
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8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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