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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Dhangdhariya, Jaykishan H. Dubey, Sonam Trivedi, Hiral B. Pancha, Imran Bhatt, Jwalant K. Dave, Bharti P. Mishra, Sandhya |
| Description | Country affiliation: India Author Affiliation: Dhangdhariya JH ( Department of Life Sciences, Maharaja Krishnakumarsinhji Bhavnagar University, Bhavnagar 364002, India.); Dubey S ( Discipline of Salt & Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.); Trivedi HB ( Department of Life Sciences, Maharaja Krishnakumarsinhji Bhavnagar University, Bhavnagar 364002, India.); Pancha I ( Discipline of Salt & Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India); Bhatt JK ( Department of Life Sciences, Maharaja Krishnakumarsinhji Bhavnagar University, Bhavnagar 364002, India.); Dave BP ( Department of Life Sciences, Maharaja Krishnakumarsinhji Bhavnagar University, Bhavnagar 364002, India. Electronic address: bpd8256@gmail.com.); Mishra S ( Discipline of Salt & Marine Chemicals, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India) |
| Abstract | Oceans have significant potential to empower mankind and thus marine organisms are believed to be an enormous source for useful biomolecules. Polyhydroxyalkanoates (PHAs) are biological macromolecules that can be applied in nearly all fields. In the present study, Bacillus megaterium strain JK4h has been exploited for maximum PHB production using novel Dry Sea Mix (DSM) via Central Composite Design (CCD) of Response Surface Methodology (RSM) approach. The isolate was found to be producing 56.77% Cell Dry Weight (CDW) of PHAs within 24h, with optimized combinations of peptone, yeast extract and glucose. The PHB yield had been increased 2.61 fold compared to un-optimized experiments. The obtained PHA/PHB had been chemically characterized through Nuclear Magnetic Resonance (NMR), Fourier Transform Infrared Spectroscopy (FTIR), Thermo Gravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC). The results indicate the successful optimization for maximum production of biological macromolecule and it was found to be highly pure polyhydroxybutyrate (PHB). Thus, DSM can be served as a novel and cost effective medium for PHA production offering the use of marine resources as a 'green' sustainable alternative. |
| File Format | HTM / HTML |
| ISSN | 01418130 |
| Volume Number | 76 |
| e-ISSN | 18790003 |
| Journal | International Journal of Biological Macromolecules |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-05-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Bacillus Megaterium Metabolism Polyhydroxyalkanoates Chemistry Pharmacology Classification Genetics Calorimetry, Differential Scanning Chemical Fractionation Genes, Bacterial Nuclear Magnetic Resonance, Biomolecular Phylogeny Reproducibility Of Results Spectroscopy, Fourier Transform Infrared Thermogravimetry Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Structural Biology Molecular Biology Biochemistry |
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