NDLI: The salivary proteome in relation to oral mucositis in autologous hematopoietic stem cell transplantation recipients: a labelled and label-free proteomics approach

Content Provider	Springer Nature : BioMed Central
Author	van Leeuwen, S. J. M. Proctor, G. B. Staes, A. Laheij, A. M. G. A. Potting, C. M. J. Brennan, M. T. von Bültzingslöwen, I. Rozema, F. R. Hazenberg, M. D. Blijlevens, N. M. A. Raber-Durlacher, J. E. Huysmans, M. C. D. N. J. M.
Abstract	Background Oral mucositis is a frequently seen complication in the first weeks after hematopoietic stem cell transplantation recipients which can severely affects patients quality of life. In this study, a labelled and label-free proteomics approach were used to identify differences between the salivary proteomes of autologous hematopoietic stem cell transplantation (ASCT) recipients developing ulcerative oral mucositis (ULC-OM; WHO score ≥ 2) or not (NON-OM). Methods In the TMT-labelled analysis we pooled saliva samples from 5 ULC-OM patients at each of 5 timepoints: baseline, 1, 2, 3 weeks and 3 months after ASCT and compared these with pooled samples from 5 NON-OM patients. For the label-free analysis we analyzed saliva samples from 9 ULC-OM and 10 NON-OM patients at 6 different timepoints (including 12 months after ASCT) with Data-Independent Acquisition (DIA). As spectral library, all samples were grouped (ULC-OM vs NON-OM) and analyzed with Data Dependent Analysis (DDA). PCA plots and a volcano plot were generated in RStudio and differently regulated proteins were analyzed using GO analysis with g:Profiler. Results A different clustering of ULC-OM pools was found at baseline, weeks 2 and 3 after ASCT with TMT-labelled analysis. Using label-free analysis, week 1–3 samples clustered distinctly from the other timepoints. Unique and up-regulated proteins in the NON-OM group (DDA analysis) were involved in immune system-related processes, while those proteins in the ULC-OM group were intracellular proteins indicating cell lysis. Conclusions The salivary proteome in ASCT recipients has a tissue protective or tissue-damage signature, that corresponded with the absence or presence of ulcerative oral mucositis, respectively. Trial registration The study is registered in the national trial register (NTR5760; automatically added to the International Clinical Trial Registry Platform).
Related Links	https://bmcoralhealth.biomedcentral.com/counter/pdf/10.1186/s12903-023-03190-w.pdf
Ending Page	17
Page Count	17
Starting Page	1
File Format	HTM / HTML
ISSN	14726831
DOI	10.1186/s12903-023-03190-w
Journal	BMC Oral Health
Issue Number	1
Volume Number	23
Language	English
Publisher	BioMed Central
Publisher Date	2023-07-07
Access Restriction	Open
Subject Keyword	Dentistry Oral and Maxillofacial Surgery Oral mucositis Saliva TMT-labelled proteomics Label-free quantification Autologous hematopoietic stem cell transplantation Multiple myeloma
Content Type	Text
Resource Type	Article
Subject	Dentistry
Journal Impact Factor	2.6/2023
5-Year Journal Impact Factor	3.2/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Significant salivary changes in relation to oral mucositis following autologous hematopoietic stem cell transplantation.

Microbial changes in relation to oral mucositis in autologous hematopoietic stem cell transplantation recipients

A randomized phase II study of acyclovir for the prevention of chemotherapy-induced oral mucositis in patients undergoing autologous hematopoietic stem cell transplantation

Salivary Osteopontin as a Potential Biomarker for Oral Mucositis

Risk factors for oral mucositis in paediatric oncology patients receiving alkylant chemotherapy

Autologous Hematopoietic Stem Cell Transplantation in Dialysis-Dependent Myeloma Patients.

Hepatitis B-related events in autologous hematopoietic stem cell transplantation recipients

Therapeutic effects of autologous hematopoietic stem cell transplantation in multiple myeloma patients.

Outcomes of Non-Cryopreserved Versus Cryopreserved Peripheral Blood Stem Cells for Autologous Stem Cell Transplantation in Multiple Myeloma.

The salivary proteome in relation to oral mucositis in autologous hematopoietic stem cell transplantation recipients: a labelled and label-free proteomics approach

Similar Documents

Significant salivary changes in relation to oral mucositis following autologous hematopoietic stem cell transplantation.

Microbial changes in relation to oral mucositis in autologous hematopoietic stem cell transplantation recipients

A randomized phase II study of acyclovir for the prevention of chemotherapy-induced oral mucositis in patients undergoing autologous hematopoietic stem cell transplantation

Salivary Osteopontin as a Potential Biomarker for Oral Mucositis

Risk factors for oral mucositis in paediatric oncology patients receiving alkylant chemotherapy

Autologous Hematopoietic Stem Cell Transplantation in Dialysis-Dependent Myeloma Patients.

Hepatitis B-related events in autologous hematopoietic stem cell transplantation recipients

Therapeutic effects of autologous hematopoietic stem cell transplantation in multiple myeloma patients.

Outcomes of Non-Cryopreserved Versus Cryopreserved Peripheral Blood Stem Cells for Autologous Stem Cell Transplantation in Multiple Myeloma.

The salivary proteome in relation to oral mucositis in autologous hematopoietic stem cell transplantation recipients: a labelled and label-free proteomics approach