NDLI: Expression of UNC93A induced by CpG-DNA-liposome complex in mice

Content Provider	Springer Nature Link
Author	Kwon, Hyung Joo Kim, Te Ha Kim, Dongbum Lee, Younghee
Copyright Year	2014
Abstract	UNC93B1 is involved in the delivery of nucleotide-sensing Toll-like receptors (TLR) including TLR9 to endolysosomes. However, possible functions of UNC93A, another member of the UNC-93 superfamily, have not been studied in the context of TLR signaling. Here, we injected naked CpG-ODNs or liposome-encapsulated CpG-ODN (Lipoplex(O)) into the BALB/c mouse peritoneal cavity and investigated expression of mouse UNC93B1 and UNC93A genes in the peritoneal cells. UNC93A mRNA expression was increased by Lipoplex(O) in a time-dependent manner, whereas the expression level of UNC93B1 was not changed. To evaluate whether the expression of UNC93A involves TLR9, TLR9 knock out (TLR-/-) mice were injected with Lipoplex(O) or LipoplexGC(O), and the peritoneal cells were analyzed. The expression of UNC93A was not induced by Lipoplex(O) in TLR9-/- mice. These results suggest that UNC93A is closely associated with TLR9 signaling induced by Lipoplex(O) in the peritoneal cells in vivo. Primary cells including peritoneal cells stimulated with CpG-ODNs, Lipoplex(O), and several cytokines in vitro were prepared. However, UNC93A expression was not induced by any stimulation. To identify cellular localization of UNC93A, human embryonic kidney 293 cells stably expressing UNC93A were established and analyzed by confocal microscopy. The human and mouse UNC93A proteins were detected in the cytoplasm. Further investigation of the UNC93A function related with the CpG-DNA-mediated immune response may provide information to support efficient application of CpG-DNA immunotherapeutics.
Starting Page	281
Ending Page	287
Page Count	7
File Format	PDF
ISSN	17382203
Journal	Journal of the Korean Society for Applied Biological Chemistry
Volume Number	57
Issue Number	3
e-ISSN	2234344X
Language	English
Publisher	The Korean Society for Applied Biological Chemistry
Publisher Date	2014-05-25
Publisher Place	Seoul
Access Restriction	Subscribed
Subject Keyword	CpG-DNA-liposome complex innate immunity TLR9 Applied Microbiology Biological Techniques CpG-DNA UNC93A Bioorganic Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry, Genetics and Molecular Biology

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