NDLI: Thrombomodulin mediates the migration of cervical cancer cells through the regulation of epithelial

Content Provider	Springer Nature Link
Author	Tai, Cheng Jeng Cheng, Chao Wen Su, Hou Yu Chen, Wei Yu Wu, Chun Te Lin, Feng Yen Wang, Chien Kai Tai, Chen Jei Wei, Po Li
Copyright Year	2013
Abstract	Thrombomodulin (TM) has been shown to regulate many physiological and pathological processes, including inflammation, thrombosis, and tumor progression. TM is also a natural anticoagulant that maintains circulatory homeostasis in endothelial cells. However, little is known regarding the role of TM in the progression and metastasis of cervical cancer. TM-specific RNA interference and a cDNA expression vector were used to manipulate TM expression in cervical cancer cells. Cell growth and cell migration were evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, transwell migration assays, and a biosensor system. TM silencing did not affect the growth rate of the cells. However, cell migration was dramatically enhanced after silencing of TM in HeLa cells. The overexpression of TM in cervical cancer cells only slightly influenced their proliferative capacity. After overexpression of TM in HeLa cells, their migratory capability was suppressed. Furthermore, we found that the decreased expression of E-cadherin and increase of zeb-1 and snail expression in TM-silenced cells which may be correlated with the results of knocking-down TM increases the migratory ability in this study. Our results demonstrate that TM may slightly regulate the growth but played the important role in the migratory ability of cervical cancer cells, suggesting that TM could potentially serve as a novel prognostic and therapeutic target in cervical cancer.
Starting Page	47
Ending Page	54
Page Count	8
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	35
Issue Number	1
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-07-24
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Thrombomodulin Cervical cancer Migration EMT Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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