NDLI: MiR-148a regulates the growth and apoptosis in pancreatic cancer by targeting CCKBR and Bcl-2

Content Provider	Springer Nature Link
Author	Zhang, Rui Li, Min Zang, Wenqiao Chen, Xudong Wang, Yuanyuan Li, Ping Du, Yuwen Zhao, Guoqiang Li, Li
Copyright Year	2013
Abstract	Our previous studies have revealed that miR-148a is downregulated in pancreatic cancer. Bioinformatics analysis has shown cholecystokinin-B receptor (CCKBR) and B cell lymphoma (Bcl-2) to be potential targets of miR-148a. But the pathophysiologic role of miR-148a and its relevance to the growth and development of pancreatic cancer are yet to be investigated. The purpose of this study is to elucidate the molecular mechanisms where miR-148a acts as a tumor suppressor in pancreatic cancer. Our results showed significant downregulation of miR-148a in 28 pancreatic cancer tissue samples and five pancreatic cancer cell lines, compared with their non-tumor counterparts by qRT-PCR. MiR-148a was found to not only inhibit the proliferation of pancreatic cancer cells (PANC-1 and AsPC-1) in vitro by MTT assay and colony formation assay, but also to promote cells apoptosis in vitro by Annexin V-FITC apoptosis detection and caspase activity assay. Using western blot and luciferase activity assay, CCKBR and Bcl-2 were identified as targets of miR-148a. Moreover, we also found that the expression of Bcl-2 lacking in 3′UTR could abrogate the pro-apoptosis function of miR-148a. These findings suggest the importance of miR-148a’s targeting of CCKBR and Bcl-2 in the regulation of pancreatic cancer growth and apoptosis.
Starting Page	837
Ending Page	844
Page Count	8
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	35
Issue Number	1
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-08-23
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	miR-148a Pancreatic cancer Cholecystokinin-B receptor Bcl-2 Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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