NDLI: MiR-7-5p is frequently downregulated in glioblastoma microvasculature and inhibits vascular endothelial cell proliferation by targeting RAF1

Content Provider	Springer Nature Link
Author	Liu, Zhiguo Liu, Yuguang Li, Lianling Xu, Zhenkuan Bi, Baibin Wang, Yunyan Li, Jian Yi
Copyright Year	2014
Abstract	The aberrant expression of microRNAs (miRNAs) is always associated with tumor development and progression. Microvascular proliferation is one of the unique pathologic features of glioblastoma (GBM) . In this study, the microvasculature from GBM or normal brain tissue derived from neurosurgeries was purified and total RNA was isolated from purified microvasculature. The difference of miRNA expression profiles between glioblastoma microvasculature and normal brain capillaries was investigated. It was found that miR-7-5p in GBM microvessels was significantly reduced compared with that in normal brain capillaries. In the in vitro experiments, overexpression of miR-7-5p significantly inhibited human umbilical vein endothelial cell proliferation. Forced expression of miR-7-5p in human umbilical vein endothelial cells in vitro significantly reduced the protein level of RAF1 and repressed the activity of the luciferase, a reporter vector carrying the 3′-untranslated region of RAF1. These findings indicate that RAF1 is one of the miR-7-5p target genes. Furthermore, a significant inverse correlation between miR-7-5p expression and RAF1 protein level in GBM microvasculature was found. These data suggest that miR-7-5p functions as a tumor suppressor gene to regulate GBM microvascular endothelial cell proliferation potentially by targeting the RAF1 oncogene, implicating an important role for miR-7-5p in the pathogenesis of GBM. It may serve as a guide for the antitumor angiogenesis drug development.
Starting Page	10177
Ending Page	10184
Page Count	8
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	35
Issue Number	10
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-07-16
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Microvasculature MiR-7-5p Glioblastoma RAF1 Proliferation Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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