NDLI: Inhibitory effects of sea buckthorn procyanidins on fatty acid synthase and MDA-MB-231 cells

Content Provider	Springer Nature Link
Author	Wang, Yi Nie, Fangyuan Ouyang, Jian Wang, Xiaoyan Ma, Xiaofeng
Copyright Year	2014
Abstract	Fatty acid synthase (FAS) is overexpressed in many human cancers including breast cancer and is considered to be a promising target for therapy. Sea buckthorn has long been used to treat a variety of maladies. Here, we investigated the inhibitory effect of sea buckthorn procyanidins (SBPs) isolated from the seeds of sea buckthorn on FAS and FAS overexpressed human breast cancer MDA-MB-231 cells. The FAS activity and FAS inhibition were measured by a spectrophotometer at 340 nm of nicotinamide adenine dinucleotide phosphate (NADPH) absorption. We found that SBP potently inhibited the activity of FAS with a half-inhibitory concentration (IC$_{50}$) value of 0.087 μg/ml. 3-4,5-Dimethylthiazol-2-yl-2,3-diphenyl tetrazolium bromide (MTT) assay was used to test the cell viability. SBP reduced MDA-MB-231 cell viability with an IC$_{50}$ value of 37.5 μg/ml. Hoechst 33258/propidium iodide dual staining and flow cytometric analysis showed that SBP induced MDA-MB-231 cell apoptosis. SBP inhibited intracellular FAS activity with a dose-dependent manner. In addition, sodium palmitate could rescue the cell apoptosis induced by SBP. These results showed that SBP was a promising FAS inhibitor which could induce the apoptosis of MDA-MB-231 cells via inhibiting FAS. These findings suggested that SBP might be useful for preventing or treating breast cancer.
Starting Page	9563
Ending Page	9569
Page Count	7
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	35
Issue Number	10
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-06-24
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Fatty acid synthase Inhibitor Sea buckthorn Procyanidins MDA-MB-231 cells Cell apoptosis Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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