NDLI: Characterization of Lin$^{−}$ALDH$^{bright}$ population using Ehrlich ascites tumor cells in mice

Content Provider	Springer Nature Link
Author	Yalçintepe, Leman Altinel, Pinar Albeniz, Işil Yilmaz, Abdullah Nurten, Rustem
Copyright Year	2014
Abstract	Cancer stem cells (CSCs)/tumor initiating cells have been shown to exist in recent studies; however, it is challenging to isolate these cells. The latest evidence suggests that elevated aldehyde dehydrogenase (ALDH) activity is a hallmark of CSCs. In this study, mice implanted with Ehrlich ascites tumor (EAT) cells were used to isolate cancer stem cells. Femoral bone marrow aspirations were performed 15 days after the injection of EAT cells and Lin$^{−}$ALDH$^{bright}$ and Lin$^{−}$ALDH$^{low}$ cell populations were isolated. Lin$^{−}$ALDH$^{bright}$ cells isolated from EAT-bearing mice accounted for 11.08 ± 10.52 % of all the Lin$^{−}$ cell population. Analysis of hematopoietic stem cell markers showed that Sca-1, c-kit, and CD38 were expressed higher in the Lin$^{−}$ALDH$^{bright}$ population compared with Lin$^{−}$ALDH$^{low}$. The Lin$^{−}$ALDH$^{bright}$ population expressed P-glycoprotein, a product of the multidrug resistance (MDR) gene. P-gp activity measured by rhodamine 123 (Rh123) and blocked by verapamil. Among the cells treated with doxorubicin for 48 h, the Lin$^{−}$ALDH$^{bright}$ cell groups were more resistant and had higher overexpression of Bcl-2 protein than Lin$^{−}$ALDH$^{low}$.
Starting Page	10363
Ending Page	10373
Page Count	11
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	35
Issue Number	10
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-07-23
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Stem cell Cancer stem cell ALDH Drug resistance Ehrlich ascites tumor Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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