NDLI: Transcriptome analysis of the cancer/testis genes, DAZ1, AURKC, and TEX101, in breast tumors and six breast cancer cell lines

Content Provider	Springer Nature Link
Author	Mobasheri, Maryam Beigom Shirkoohi, Reza Zendehdel, Kazem Jahanzad, Issa Talebi, Saeid Afsharpad, Mandana Modarressi, Mohammad Hossein
Copyright Year	2015
Abstract	Breast cancer is the most frequent cancer with second mortality rate in women worldwide. Lack of validated biomarkers for early detection of breast cancer to warranty the diagnosis and effective treatments in early stages has directed to the new therapeutic approach. Cancer/testis antigens which have restricted normal expression in testis and aberrant expression in different cancers are promising targets for generating cancer vaccines, monoclonal antibodies, or dendritic cell-based immunotherapy. In this context, we investigated the expression of two known cancer testis genes, Aurora kinase C (AURKC) and testis expressed 101 (TEX101), and one new candidate, deleted in azoospermia 1 (DAZ1), in six breast cancer cell lines including two ductal carcinomas, T47D and BT-474, and four adenocarcinomas, MDA-MB-231, MDA-MB-468, MCF7, and SKBR3 as well as 50 breast cancer tumors in comparison to normal mammary epithelial cells using quantitative real-time reverse transcription PCR (RT-PCR). Results showed significant overexpression (p = 0.000) of all three genes in BT474, DAZ1 in MDA-MB-231, and AURKC and DAZ1 in SKBR3 and significant downregulation (p = 0.000) of AURKC in MCF7 cell line relative to normal breast epithelial cells. Breast tumors showed significant overexpression of AURKC in comparison to normal breast tissues (p = 0.016). The results are noticeable especially in the case of AURKC; however, there is a little knowledge about the nature, causes, consequences, and effects of cancer/testis antigens activation in different cancers. It is suggested that AURKC has effects on cell division via its serin/threonin kinases activity and organizing microtubules in relation to centrosome/spindle function during mitosis.
Starting Page	8201
Ending Page	8206
Page Count	6
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	36
Issue Number	10
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-05-21
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	TEX101 AURKC DAZ1 Breast cancer Immunotherapy Targeted therapy Cancer/testis genes Breast cancer cell lines Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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