NDLI: Overexpression of the ∆Np73 isoform is associated with centrosome amplification in brain tumor cell lines

Content Provider	Springer Nature Link
Author	Mikulenkova, Erika Neradil, Jakub Zitterbart, Karel Sterba, Jaroslav Veselska, Renata
Copyright Year	2015
Abstract	The p73 protein is a member of the p53 family, and this protein is known to be essential for the maintenance of genomic stability, DNA repair, and apoptosis regulation. Transcription from two promoters leads to two main N-terminal isoforms: the TAp73 isoform is reported to have tumor suppressor function, whereas the ΔNp73 isoform likely has oncogenic potential. The present study is focused on the investigation of a possible role of both these p73 N-terminal isoforms in the process of centrosome amplification. HGG-02 and GM7 glioblastoma cell lines and the Daoy medulloblastoma cell line were used in this study. The cells were analyzed using indirect immunofluorescence to determine TAp73 and ΔNp73 expression patterns and possible co-localization with the BubR1 protein, as well as the number of centrosomes. A transiently transfected GM7 cell line was used to verify the results concerning the N-terminal isoforms in relation to centrosome amplification. We found that increased immunoreactivity for the ΔNp73 isoform is associated with the occurrence of an abnormal number of centrosomes in particular cells. Using the transiently transfected GM7 cell line, we confirmed that centrosome amplification is present in cells with overexpression of the ΔNp73 isoform. In contrast, the immunoreactivity for the TAp73 isoform was weak or medium in most of the cells with an aberrant number of centrosomes. To determine the putative counterpart of the p73 N-terminal isoforms among spindle assembly checkpoint (SAC) proteins, we also evaluated possible interactions between the N-terminal isoforms and BubR1 protein, but no co-localization of these proteins was observed.
Starting Page	7483
Ending Page	7491
Page Count	9
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	36
Issue Number	10
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-04-25
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Centrosome amplification Medulloblastoma Glioblastoma multiforme ΔNp73 TAp73 BubR1 Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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