NDLI: Identification and phylogenetic characterization of novel Lactobacillus plantarum species and their metabolite profiles in grass silage

Content Provider	Springer Nature Link
Author	Valan Arasu, Mariadhas Jung, Min Woong Kim, Da Hye Park, Hyung Soo Ilavenil, Soundarrajan Al Dhabi, Naif Abdullah Choon Choi, Ki
Copyright Year	2014
Abstract	Italian ryegrass (IRG), barley, and rye are types of forage that are difficult to ensile with an assurance of good quality. Therefore, the addition of lactic acid bacteria (LAB) is the best way to enhance the preservation of this silage. However, applications of LAB have been impeded due to its poor growth characteristics, the sudden decline in pH, and other non-beneficial microbial growth associated with its presence. To overcome these limitations, a new Lactobacillus sp. KCC-10 and KCC-19 strain was isolated from well-fermented IRG silage samples. Biochemical and physiological studies revealed that the strains were Gram-positive, catalase-negative, and produced gas from glucose, and produced more lactic acid in fermentation. The 16S rRNA gene-based phylogenetic affiliation was determined by using bioinformatic tools that identified Lactobacillus sp. KCC-10 and KCC-19 with 100 % sequence similarity to Lactobacillus plantarum. Novel L. plantarum strains were deposited in the Korean Collection for Type Cultures under the accession numbers KACC 91785P and KACC 91758P, respectively. The shake-flask cultivation of these new strains under aerobic, microaerobic, and anaerobic conditions showed a higher specific growth rate than that achieved using the well-studied L. plantarum KACC 91016 and KACC 91096 on MRS broth and grass juice. Lactic acid was detected as the dominant organic acid in IRG (78.45 mM), barley (51.28 mM), corn (16.28 mM), and rice paddy (11.05 mM), followed by acetic acid and succinic acid. The KCC-10 in the silage was observed to increase from 2.4 × 10$^{5}$ CFU/g per sample at day 0 to 0.58, 0.60, and 0.59 × 10$^{9}$ CFU/g at day 5 for IRG, barley, and rye, respectively. The growth of KCC-10 and KCC-19 in all the silages decreased, as the storage period increased from 5 to 50 days. Whereas, KCC-19 was noted to increase from 2.7 × 10$^{5}$ CFU/g per sample at day 0 to 0.71, 0.72, and 0.711 × 10$^{9}$ CFU/g at day 5 for IRG, barley, and rye. Among the total organic acids, lactic acid was detected as the dominant acid present in IRG, barley, and rye silages. From these results, we concluded that strains KCC-10 and KCC-19 can be used as appropriate inoculants to prolong the stability of silage and fermentation quality.
Starting Page	15
Ending Page	25
Page Count	11
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	65
Issue Number	1
e-ISSN	18692044
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-02-25
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	L. plantarum 16S rRNA gene Shake-flask cultivation Lactic acid Silage fermentation Microbiology Microbial Genetics and Genomics Microbial Ecology Fungus Genetics Medical Microbiology Applied Microbiology
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Isolation and preliminary characterization of Lactobacillus plantarum bacteriophages from table olive fermentation

Characteristics of lactic acid bacteria, microbial community and fermentation dynamics of native grass silage prepared in Inner Mongolian Plateau

Identification and characterization of lactic acid bacteria isolated from tomato pomace

Sequence analysis of 16S rRNA gene and 16S–23S rRNA gene intergenic spacer region for differentiation of probioticsLactobacillus strains isolated from the gastrointestinal tract of chicken

Comparison of 16S rRNA, 23S rRNA andgyrB genes sequences in phylogenetic relationships ofShigella isolates from Iran

Isolation of probiotic lactobacilli strains harboring l-asparaginase and arginine deiminase genes from human infant feces for their potential application in cancer prevention

Activity against plant pathogenic fungi of Lactobacillus plantarum IMAU10014 isolated from Xinjiang koumiss in China

Enhanced D-lactic acid production from renewable resources using engineered Lactobacillus plantarum

Production of lactic acid from cheese whey by batch cultures of Lactobacillus helveticus

Identification and phylogenetic characterization of novel Lactobacillus plantarum species and their metabolite profiles in grass silage

Similar Documents

Isolation and preliminary characterization of Lactobacillus plantarum bacteriophages from table olive fermentation

Characteristics of lactic acid bacteria, microbial community and fermentation dynamics of native grass silage prepared in Inner Mongolian Plateau

Identification and characterization of lactic acid bacteria isolated from tomato pomace

Sequence analysis of 16S rRNA gene and 16S–23S rRNA gene intergenic spacer region for differentiation of probioticsLactobacillus strains isolated from the gastrointestinal tract of chicken

Comparison of 16S rRNA, 23S rRNA andgyrB genes sequences in phylogenetic relationships ofShigella isolates from Iran

Isolation of probiotic lactobacilli strains harboring l-asparaginase and arginine deiminase genes from human infant feces for their potential application in cancer prevention

Activity against plant pathogenic fungi of Lactobacillus plantarum IMAU10014 isolated from Xinjiang koumiss in China

Enhanced D-lactic acid production from renewable resources using engineered Lactobacillus plantarum

Production of lactic acid from cheese whey by batch cultures of Lactobacillus helveticus

Identification and phylogenetic characterization of novel Lactobacillus plantarum species and their metabolite profiles in grass silage