NDLI: Characterization of the second and third steps in the enzymatic pathway for microcystin-RR biodegradation by Sphingopyxis sp. USTB-05

Content Provider	Springer Nature Link
Author	Wang, Huasheng Yan, Hai Ma, Song Liu, Xiaolu Yin, Chunhua Wang, Hui Xu, Qianqian Lv, Le
Copyright Year	2014
Abstract	Microcystin-RR (MC-RR) is a dominant variant of microcystin (MC) worldwide. We previously isolated Sphingopyxis sp. USTB-05, which can efficiently biodegrade MC-RR. At least three enzymes encoded by MC-degrading genes are involved. Based on the successful expression of the first gene USTB-05-A, the second (USTB-05-B, KC513423) and third (USTB-05-C, KC573527) genes involved in the biodegradation of MC-RR were further cloned from Sphingopyxis sp. USTB-05 and expressed in Escherichia coli BL21 (DE3). After purification, the MC-degrading enzymes encoded by these genes were used for the catalytic degradation of MC-RR. The results demonstrated that the second enzyme encoded by USTB-05-B could convert linear MC-RR to a tetrapeptide by breaking the Ala–Arg bond. The third enzyme encoded by USTB-05-C could cleave Adda-Glu peptide bonds of both linear MC-RR and the tetrapeptide of Adda-Glu-Mdha-Ala, producing Adda as their common product. These findings will help better understand the biodegradation mechanism of MCs by Sphingopyxis sp. USTB-05.
Starting Page	495
Ending Page	502
Page Count	8
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	65
Issue Number	1
e-ISSN	18692044
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-04-27
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Microcystin-RR Sphingopyxis sp. USTB-05 Cloning and expression of MC-degrading genes Biodegradation pathway Microbiology Microbial Genetics and Genomics Microbial Ecology Fungus Genetics Medical Microbiology Applied Microbiology
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

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