NDLI: Physical and chemical stress on Serratia marcescens and studies on prodigiosin pigment production

Content Provider	Springer Nature Link
Author	El Bialy, Heba A. Abou El Nour, Salwa A.
Copyright Year	2014
Abstract	Bio-pigments play a very important role in many industries such as food, feed, cosmetics and pharmaceutical products. The red pigment prodigiosin produced by Serratia marcescens possesses a lot of medical benefits. The employment of ultraviolet (UV) radiation and ethyl methanesulfonate (EMS) to induce variants with the enhanced pigmentation of S. marcescens is the main scope of this study. Also, the antimicrobial activity and the cytotoxicity properties of prodigiosin were investigated. Subsequent to physical and chemical stress induced on S. marcescens, many variants are selected. They showed different patterns in growth at 38 °C, salinity tolerance, emulsification and hemolytic activities compared to the parent S. marcescens. The EMS-variant (S$_{26}$) produced prodigiosin eight-fold higher than that of the parent strain. Three new media are described for S. marcescens’s growth and pigmentation. They are based on the market-refused squash seeds; sunflower seeds and watermelon seeds. The present study modifies and designs a new procedure for prodigiosin extraction using small volumes of bacterial cultures. Prodigiosin was purified using column chromatography; it was found that prodigiosin is susceptible to non-polar solvents. Crude prodigiosin extracted from variant (S$_{26}$) is stable at alkaline pH and its thermal stability is up to 80 °C. The experiment of testing the dyeing properties of prodigiosin is outlined and its results are deeply encouraging for the use of this interesting pigment for ultimate medical applications.
Starting Page	59
Ending Page	68
Page Count	10
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	65
Issue Number	1
e-ISSN	18692044
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-03-27
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Serratia marcescens Prodigiosin pigment EMS UV Textile dyeing Microbiology Microbial Genetics and Genomics Microbial Ecology Fungus Genetics Medical Microbiology Applied Microbiology
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

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