NDLI: Enhanced D-lactic acid production from renewable resources using engineered Lactobacillus plantarum

Content Provider	Springer Nature Link
Author	Zhang, Yixing Vadlani, Praveen V. Kumar, Amit Hardwidge, Philip R. Govind, Revathi Tanaka, Tsutomu Kondo, Akihiko
Copyright Year	2015
Abstract	D-lactic acid is used as a monomer in the production of poly-D-lactic acid (PDLA), which is used to form heat-resistant stereocomplex poly-lactic acid. To produce cost-effective D-lactic acid by using all sugars derived from biomass efficiently, xylose-assimilating genes encoding xylose isomerase and xylulokinase were cloned into an L-lactate-deficient strain, Lactobacillus plantarum. The resulting recombinant strain, namely L. plantarum NCIMB 8826 ∆ldhL1-pLEM-xylAB, was able to produce D-lactic acid (at optical purity >99 %) from xylose at a yield of 0.53 g g−1. Simultaneous utilization of glucose and xylose to produce D-lactic acid was also achieved by this strain, and 47.2 g L−1 of D-lactic acid was produced from 37.5 g L−1 glucose and 19.7 g L−1 xylose. Corn stover and soybean meal extract (SBME) were evaluated as cost-effective medium components for D-lactic acid production. Optimization of medium composition using response surface methodology resulted in 30 % reduction in enzyme loading and 70 % reduction in peptone concentration. In addition, we successfully demonstrated D-lactic acid fermentation from corn stover and SBME in a fed-batch fermentation, which yielded 61.4 g L−1 D-lactic acid with an overall yield of 0.77 g g−1. All these approaches are geared to attaining high D-lactic acid production from biomass sugars to produce low-cost, highly thermostable biodegradable plastics.
Starting Page	279
Ending Page	288
Page Count	10
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	100
Issue Number	1
e-ISSN	14320614
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-10-03
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Lactic acid Lactobacillus plantarum Corn Stover Response surface methodology Xylose fermentation Microbiology Microbial Genetics and Genomics Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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