NDLI: In vitro classical conditioning of the turtle eyeblink reflex: approaching cellular mechanisms of acquisition

Please wait, while we are loading the content...

In vitro classical conditioning of the turtle eyeblink reflex: approaching cellular mechanisms of acquisition

Content Provider	Springer Nature Link
Author	Keifer, Joyce
Copyright Year	2003
Abstract	The classically conditioned eyeblink reflex is the best studied model for understanding the neural mechanisms that underlie learning and memory. Here, data from an in vitro model of the conditioned eyeblink reflex are summarized with the aim of shedding some light on potential cellular mechanisms that may underlie eyeblink classical conditioning. An isolated brainstem-cerebellum preparation from turtles was developed in which to study the synaptic circuitry of pathways involving the cerebellum, red nucleus and brainstem nuclei. A neural correlate of an eyeblink response recorded in the abducens nerve can be conditioned entirely in vitro by pairing trigeminal and auditory nerve stimulation. Conditioned abducens nerve responses (CRs) are not generated or sustained by unpaired stimuli and their long latencies, on the order of hundreds of milliseconds, support the Interpretation that the CRs are not unconditioned responses. Ablation experiments show that CRs can be generated in brainstem preparations lacking a cerebellum or the medulla. However, the timing of the CRs are disrupted by removal of the cerebellar circuitry. Thus, a highly reduced in vitro brainstem preparation demonstrates acquisition of CRs but poor timing features. Recent experiments have focused on elucidating cellular mechanisms for CR acquisition in the brainstem blink circuitry. These studies show that NMDA-mediated synaptic mechanisms are required to generate CRs and that the level of conditioning is associated with the upregulation of GluR4-containing AMPA receptors in the abducens motor nuclei. Data from immunocytochemistry and physiological experiments using the calcium/calmodulin-dependent protein kinase II (CaMKII) inhibitor KN-93 suggest that CaMKII does not have a key role in mediating the induction or expression of abducens nerve CRs. It is hypothesized that GluR4-containing AMPA receptors In the abducens motor nuclei are targeted to auditory nerve synapses by an NMDA receptor-dependent process to strengthen the CS input during conditioning which results in the generation of CRs. Future studies will examine the synaptic localization of GluR4 and potential signal transduction pathways involved in in vitro conditioning. Moreover, the role feedback loops through the cerebellum and their role in CR timing will be a key issue to address using this preparation.
Starting Page	55
Ending Page	61
Page Count	7
File Format	PDF
ISSN	14734222
Journal	The Cerebellum
Volume Number	2
Issue Number	1
e-ISSN	14734230
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Classical conditioning eyeblink turtle In vitro NMDA receptors Neurosciences Neurobiology Neurology
Content Type	Text
Resource Type	Article
Subject	Neurology Neurology (clinical)

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The effects of ethanol on the developing cerebellum and eyeblink classical conditioning

Spike-Coding Mechanisms of Cerebellar Temporal Processing in Classical Conditioning and Voluntary Movements

Golgi Cell Activity During Eyeblink Conditioning in Decerebrate Ferrets

Asymmetries in Cerebellar Plasticity and Motor Learning

Eyeblink Conditioning in Healthy Adults: A Positron Emission Tomography Study

The involvement of the human cerebellum in eyeblink conditioning

Computational Models of Timing Mechanisms in the Cerebellar Granular Layer

Report on a workshop concerning the cerebellum and motor learning, held in St Louis October 2004

Dynamic Changes in the Cerebellar-Interpositus/Red-Nucleus-Motoneuron Pathway During Motor Learning

In vitro classical conditioning of the turtle eyeblink reflex: approaching cellular mechanisms of acquisition

Similar Documents

The effects of ethanol on the developing cerebellum and eyeblink classical conditioning

Spike-Coding Mechanisms of Cerebellar Temporal Processing in Classical Conditioning and Voluntary Movements

Golgi Cell Activity During Eyeblink Conditioning in Decerebrate Ferrets

Asymmetries in Cerebellar Plasticity and Motor Learning

Eyeblink Conditioning in Healthy Adults: A Positron Emission Tomography Study

The involvement of the human cerebellum in eyeblink conditioning

Computational Models of Timing Mechanisms in the Cerebellar Granular Layer

Report on a workshop concerning the cerebellum and motor learning, held in St Louis October 2004

Dynamic Changes in the Cerebellar-Interpositus/Red-Nucleus-Motoneuron Pathway During Motor Learning

In vitro classical conditioning of the turtle eyeblink reflex: approaching cellular mechanisms of acquisition