NDLI: Structural Basis for Differences in Dynamics Induced by Leu Versus Ile Residues in the CD Loop of Kir Channels

Content Provider	Springer Nature Link
Author	Lü, Shouqin An, Hailong Zhang, Hailin Long, Mian
Copyright Year	2015
Abstract	The effect of the conserved Leu/Ile site in the CD loop on the gating dynamics of Kir channels and corresponding micro-structural mechanism remains unclear. Molecular dynamics simulations were performed to investigate the structural mechanism of chicken Kir2.2. Compared to WT, the I223L mutant channel bound to PIP$_{2}$ more strongly, was activated more rapidly, and maintained the activation state more stably after PIP$_{2}$ dissociation. Cellular electrophysiology assays of mouse Kir2.1 and human Kir2.2 indicated that, consistent with simulations, the Leu residue increased the channel responses to PIP$_{2}$ through increased binding affinity and faster activation kinetics, and the deactivation kinetics decreased upon PIP$_{2}$ inhibition. The Ile residue induced the opposite responses. This difference was attributed to the distinct hydrophobic side chain symmetries of Leu and Ile; switching between these residues caused the interaction network to redistribute and offered effective conformation transduction in the Leu systems, which had more rigid and independent subunits.
Starting Page	5948
Ending Page	5961
Page Count	14
File Format	PDF
ISSN	08937648
Journal	Molecular Neurobiology
Volume Number	53
Issue Number	9
e-ISSN	15591182
Language	English
Publisher	Springer US
Publisher Date	2015-10-31
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Inward-rectifier potassium channel Molecular dynamics simulations Conformational change Patch clamp Structure-function relationship Neurosciences Neurobiology Cell Biology Neurology
Content Type	Text
Resource Type	Article
Subject	Neurology Cellular and Molecular Neuroscience

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Structural Basis for Differences in Dynamics Induced by Leu Versus Ile Residues in the CD Loop of Kir Channels

Phosphoinositide Regulation of Inward Rectifier Potassium (Kir) Channels

Inward rectifier potassium (Kir) current in dopaminergic periglomerular neurons of the mouse olfactory bulb

Permeation properties of inward-rectifier potassium channels and their molecular determinants.

Inward-rectifier potassium in basolateral membranes of frog skin epithelium channels.

Defining how multiple lipid species interact with inward rectifier potassium (Kir2) channels.

Conformational Dynamics of the Ligand-Binding Domain of Inward Rectifier K Channels as Revealed by Molecular Dynamics Simulations: Toward an Understanding of Kir Channel Gating

Functional consequences of Kir2.1/Kir2.2 subunit heteromerization

Single Channel Studies of Inward Rectifier Potassium Channel Regulation by Muscarinic Acetylcholine Receptors

Structural Basis for Differences in Dynamics Induced by Leu Versus Ile Residues in the CD Loop of Kir Channels

Similar Documents

Structural Basis for Differences in Dynamics Induced by Leu Versus Ile Residues in the CD Loop of Kir Channels

Phosphoinositide Regulation of Inward Rectifier Potassium (Kir) Channels

Inward rectifier potassium (Kir) current in dopaminergic periglomerular neurons of the mouse olfactory bulb

Permeation properties of inward-rectifier potassium channels and their molecular determinants.

Inward-rectifier potassium in basolateral membranes of frog skin epithelium channels.

Defining how multiple lipid species interact with inward rectifier potassium (Kir2) channels.

Conformational Dynamics of the Ligand-Binding Domain of Inward Rectifier K Channels as Revealed by Molecular Dynamics Simulations: Toward an Understanding of Kir Channel Gating

Functional consequences of Kir2.1/Kir2.2 subunit heteromerization

Single Channel Studies of Inward Rectifier Potassium Channel Regulation by Muscarinic Acetylcholine Receptors

Structural Basis for Differences in Dynamics Induced by Leu Versus Ile Residues in the CD Loop of Kir Channels