NDLI: From an environmental sample to a long-lasting culture: the steps to better isolate and preserve cyanobacterial strains

Content Provider	Springer Nature Link
Author	Gaget, Virginie Chiu, Yi Ting Lau, Melody Humpage, Andrew R.
Copyright Year	2016
Abstract	Cyanobacterial blooms represent a health hazard worldwide due to their toxic potential. The CyanoSurvey project was set up to evaluate the risk associated with the presence of toxigenic cyanobacteria in Australian drinking water reservoirs. It mainly targeted members of the following genera: Dolichospermum, Sphaerospermopsis, Chrysosporum, Cylindrospermopsis and Microcystis. In 2010 and 2011, 64 samples were collected nationally and 196 isolates were purified using several culture media and a concentration technique. The strains obtained were then cryopreserved using three different cryoprotectants. The efficacy of each medium, the applicability of a pre-treatment to the sample and the various cryoprotective agents were compared for several isolates of cyanobacteria. This study highlighted that the ASM-1 medium, used routinely by many laboratories for the isolation and maintenance of cyanobacteria, had the lowest efficacy for the isolation of cyanobacteria from environmental samples. Moreover, the use of a range of media combined with a concentration technique can significantly improve the chances of isolating and purifying certain groups of cyanobacteria. The use of 5 % DMSO for cryopreservation was shown to be a preferable choice of cryopreservative for most strains tested.
Starting Page	309
Ending Page	321
Page Count	13
File Format	PDF
ISSN	09218971
Journal	Journal of Applied Phycology
Volume Number	29
Issue Number	1
e-ISSN	15735176
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-09-02
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cyanobacteria Strain isolation Cryopreservation Culture collection Plant Sciences Freshwater & Marine Ecology Plant Physiology Ecology
Content Type	Text
Resource Type	Article
Subject	Plant Science Aquatic Science

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